The Role of Probiotics in Periodontal Disease

Not Applicable

Completed

• Conditions: Periodontal Diseases
• Interventions: Other: Food without additional food supplements; Dietary Supplement: AOÇ Kefir; Dietary Supplement: Probiotics

• Registration Number: NCT05211219
• Lead Sponsor: Gazi University

Brief Summary

In this randomized controlled trial, it was aimed to evaluate the effect of probiotics (chewable tablets) and kefir consumption in the initial periodontal treatment of periodontitis patients on the change of oral microbiota and treatment. In this clinical trial, 36 systemically healthy volunteers who were diagnosed with periodontitis who were admitted to Gazi University Faculty of Dentistry, Department of Periodontology were included. Besides the inclusion criteria, the nutritional habits of the patients were evaluated by questionnaire. For diagnosis, plaque index, gingival index, bleeding on probing, periodontal probing depth and clinical attachment level were examined. Patients were randomly divided into 3 groups; probiotic, kefir and control group. Following the periodontal diagnosis, clinical index records of the patients and subgingival microbial samples were obtained. The supragingival plaque sample was removed and the subgingival microbial dental plaque sample was gently taken with a sterile Gracey curette. In the same session, periodontal initial treatment was started. According to the groups, they were involved simultaneously with the treatment, kefir group was advised to consume kefir as a liquid once a day in 14 days; probiotics group was advised to consume as a chewable tablet once a day in 14 days; the control group was advised without additional food supplements. Periodontal indexes were repeated in the 1st and 3rd months. Subgingival plaque samples were taken at baseline and at 3 months and DNA sequencing was performed for Tanneralla forsythia (T. forsythia), Porphyromonas gingivalis (P. gingivalis), and Treponema denticola (T. denticola).

Detailed Description

Not available

Study Timeline

• Study Start: 2021-01-07
• Primary Completion: 2021-01-07
• Study Completion: 2021-05-28
• Study First Submitted: 2021-11-26
• Study First Submitted QC: 2022-01-16
• Study First Posted: 2022-01-27
• Status Verified: 2023-02
• Last Update Submitted: 2023-02-14
• Last Update Posted: 2023-02-15

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 36

Inclusion Criteria

1. Systemically healthy
2. Individuals over 18
3. Periodontitis (4-6 mm pockets) patients with 3 teeth in each half of the jaws except wisdom tooth
4. Non-smoker
5. Not using probiotic tablets/capsules and consuming no more than 3-4 probiotic food.

Exclusion Criteria

1. Antibiotic use within 6 months.
2. Breastfeeding or pregnancy.
3. Acute oral lesions or necrotizing ulcerative periodontitis
4. Diabetes, rheumatic fever, lung, kidney disease history and drug use that will affect periodontal tissues
5. Patients who smoke or who did not stop until last year
6. Use of probiotics tablets or capsules
7. Probiotic food consumption not more than 3-4 times a week

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Control	Food without additional food supplements	the control group was advised without additional food supplements.
Kefir	AOÇ Kefir	The kefir group was advised to consume kefir as a liquid once a day in 14 days.
Probiotics	Probiotics	The probiotics group was advised to consume a chewable tablet once a day in 14 days.

Primary Outcome Measures

Name	Time	Method
Periodontal probing depth	3rd month	the distance from the gingival margin to pocket base.
Gingival index	3rd month	It is an index that shows the severity and quantity of gingival inflammation.
Microbiological sample	3rd month	Subgingival plaque samples were taken at baseline and at 3 months and DNA sequencing was performed for Tanneralla forsythia, Porphyromonas gingivalis, and Treponema denticola.
Bleeding on probing	3rd month	Bleeding that is induced by gentle manipulation of the tissue at the depth of the gingival sulcus.
Plaque index	3rd month	It is an index that shows the amount of microbial dental plaque on the teeth.

Secondary Outcome Measures

Name	Time	Method
Clinical attachment level	3rd month	The clinical attachment level is the distance between the enamel-cementum junction and the gingival sulcus.

Trial Locations

Locations (2)

Gazi University Faculty of Dentistry; 🇹🇷 Ankara, Turkey

Tuğba Şahin; 🇹🇷 Ankara, Turkey