MicroRNA Activation of LOX-1 Mechanisms in Endometriosis
试验速览
- 阶段
- 4 期
- 状态
- 终止
- 入组人数
- 6
- 试验地点
- 1
- 主要终点
- Change in Nitric Oxide Dependent Vasodilation in the Skin
研究概览
简要总结
Endometriosis is a disorder that occurs in women. With endometriosis, tissue that should be found in the womb is found in sites outside of the womb. This disorder impairs the function of the cells that line the body's blood vessels (endothelium). The endothelium helps to control blood flow in healthy vessels. Women with this disorder have an increased risk for high blood pressure and high cholesterol. They have a higher risk for cardiovascular disease, too. With this study, we will learn how endometriosis impairs the lining of blood vessels and increases the risk for disease.
详细描述
Epidemiologic data demonstrate a clear association between endometriosis, reproductive risk factors, inflammation and cardiovascular (CV) risk. Circulating factors, Low-density lipoprotein (LDL) and oxidized LDL (oxLDL), are two of many biomarkers of cardiovascular and inflammatory disease of endometriosis. An important signaling mechanism through which circulating LDL and oxLDL act is the lectin-like oxidized LDL receptor (LOX-1). LOX-1 signal transduction functionally results in pronounced endothelial dysfunction, a hallmark of CV. We hypothesis that one factor mediating the elevated risk of cardiovascular disease in endometriosis is microRNA (miRNA) activation of LOX-1 receptor mechanisms.
Specific Aim 1. To test the hypothesis that LOX-1 receptor activation is increased leading to endothelial dysfunction in endometriosis.
Specific Aim 2. To test the hypothesis that decreased microRNAs (i.e. let7-a, let7-b, let7-g, MiR98, Mi590-p) are driving increased LOX-1 receptor expression and function in endometriosis.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Women between the ages of 18 and 45 years with endometriosis (diagnosis by prior laparoscopy by subject's own physician <5 years prior, and reported by the subject to the researchers)
排除标准
- •Use of nicotine-containing products (e.g. smoking, chewing tobacco, etc.)
- •Diabetes (HbA1C .6.5%)
- •BP>140/90
- •Taking pharmacotherapy that could alter peripheral vascular control (e.g. insulin sensitizing, cardiovascular medications)
- •Pregnancy
- •Breastfeeding
- •Taking illicit and/or recreational drugs
- •Abnormal liver function
- •Rash, skin disease, disorders of pigmentation, known skin allergies
- •Diagnosed or suspected metabolic or cardiovascular disease
- •Persistent unexplained elevations of serum transaminases
- •Known allergy to latex or investigative substances
研究组 & 干预措施
Atorvastatin
Oral atorvastatin (Lipitor) therapy (10mg/day) for seven days.
Atorvastatin acts as a systemic LOX inhibitor.
干预措施: Atorvastatin (Drug)
结局指标
主要结局
Change in Nitric Oxide Dependent Vasodilation in the Skin
时间窗: before and after intervention (7 days)
area under the curve of laser Doppler flux/mean arterial pressure \* log acetylcholine (mol/L)
次要结局
未报告次要终点
研究者
Lacy Alexander
Professor of Kinesiology, Physiology
Penn State University