Impact of Time Restricted Feeding on Daily Protein Requirements
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- University of Arkansas
- Enrollment
- 61
- Locations
- 1
- Primary Endpoint
- Whole-body protein balance
Study Overview
Brief Summary
The investigators aim to assess 24-hour protein kinetics at two different levels of protein intake: 0.8 (RDA) and 1.6g/kg/day (2RDA) during a normal dietary (TRAD) and a time restricted feeding (TRF) pattern in older adults.
Detailed Description
The investigators aim to assess 24-hour protein kinetics at two different levels of protein intake: 0.8 (RDA) and 1.6g/kg/day (2RDA) during a normal dietary (TRAD) and a time restricted feeding (TRF) pattern in older adults. Individuals in the TRF pattern will only consume food during a 6-hour eating period and fast for the remaining 18 hours. Individuals in the TRAD pattern will consume isocaloric and isonitrogenous (RDA or 2RDA) diet but consume an all-beef-breakfast. Participants will consume oral stable isotopes to measure muscle protein synthesis (MPS), whole-body muscle protein breakdown (MPB), and whole-body protein balance (WBPB). Appropriate muscle, blood samples, and urine will be collected throughout a 24-hour metabolic trial following a 2-day controlled diet.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Other
- Masking
- Double (Investigator, Outcomes Assessor)
Eligibility Criteria
- Ages
- 50 Years to 75 Years (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Men and women ages 50-75 years.
- •BMI 20-30 kg/m2 inclusive.
- •Capable of providing informed consent.
- •COVID-19 negative and/or asymptomatic
Exclusion Criteria
- •Subject who does not/will not eat animal protein sources.
- •Body mass index <20 or >
- •Hemoglobin <10g/dL at screening.
- •Platelets <150,000/uL at screening.
- •History of diabetes.
- •History of chemotherapy or radiation therapy for cancer in the 6 months prior to enrollment.
- •History of gastrointestinal bypass/reduction surgery.
- •History of a chronic inflammatory disease (e.g. what?)
- •Currently receiving androgen (e.g., testosterone) or anabolic (e.g., GH, IGF-I) therapy.
- •Currently using prescription blood thinning medications.
- •Currently using corticosteroid medications.
- •Unable or unwilling to suspend aspirin use for 7 days prior to Visit
- •Unwilling to avoid using protein or amino-acid supplements during participation.
- •Subjects who are unwilling to fast overnight.
- •Participating in >200 minutes/week of vigorous exercise and/or >4 days/week of resistance training.
Arms & Interventions
TRAD of 2RDA
A typical American dietary ingestion pattern of twice the RDA of protein/day.
Intervention: isocaloric, isonitrogenous meals (Other)
TRF of RDA
A time-restricted model of consumption of the RDA of protein/day.
Intervention: isocaloric, isonitrogenous meals (Other)
TRAD of RDA
A typical American dietary ingestion pattern of the RDA of protein/day.
Intervention: isocaloric, isonitrogenous meals (Other)
TRF of 2RDA
A time-restricted model of consumption of twice the RDA of protein/day.
Intervention: isocaloric, isonitrogenous meals (Other)
Outcomes
Primary Outcomes
Whole-body protein balance
Time Frame: 24 hours
Volume of net protein produced per unit of time
Secondary Outcomes
No secondary outcomes reported