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Pharmacokinetics and Safety of Dexamethasone Eye Drops in Preterm Infants

Phase 1
Recruiting
Conditions
Retinopathy of Prematurity
Interventions
Registration Number
NCT05387941
Lead Sponsor
Region Skane
Brief Summary

Background and study aims

When an infant is born premature, the blood vessels in the eyes have not developed fully on the retina, and can start to grow incorrectly and result in blindness. To prevent this from happening, premature infants are often screened, and treated with laser or injections into the eye to prevent retinal detachment. A new treatment strategy with steroid eye drops have been found to prevent serious blood vessel growth. The treatment is commonly used in older children and adults to treat different inflammatory conditions, but how the drop is absorbed in premature infants and if there is any risk of side-effects is poorly investigated. The aim of this study is to document how the steroid drop is absorbed and excreted in premature infants and to study if there is a risk of any side effects.

Who can participate?

Premature infants born before gestational age week 30, that undergo eye-screening at Sahlgrenska University Hospital in Gothenburg and Skånes University Hospital in Malmö and Lund or at Helsingborg Hospital, in the need for steroid eye-drop treatment against pathological vessels. It is not possible to participate if the infant has received systemic steroid treatment 2 weeks prior to the eye-drop treatment, or has an ongoing ocular infection.

What does the study involve?

The study involves blood and saliva samples according to a specific protocol designed to be able to learn about the uptake and breakdown of the steroid in premature infants. Measurements of blood pressure, growth and a few urine samples will also be collected during the treatment period usually lasting for some weeks.

At 2.5 and 5 years of age, visual acuity, refractive errors and retinal thickness measurements will be noted.

What are the possible benefits and risks of participating?

The infant will receive steroid eye-drops that have been noted to heavily reduce the number of infants that develop retinal changes that require injections or laser treatment.

The blood samples have been reduced to an absolute minimum in volume and numbers, but will entail some extra samplings from the infant.

The infant will be rigorously checked with regard to any possible side effects from the steroid treatment. Possible but unlikely side effects from the low dose in eye drops are; elevated blood pressure, retarded growth, lowered endogenous steroid production during the eye-drop treatment, increase in blood glucose, and an increase in intra-ocular pressure.

Detailed Description

Not available

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
15
Inclusion Criteria
  • Infants screened for retinopathy of prematurity (ROP) at Sahlgrenska University Hospital in Gothenburg, at Skåne University Hospital in Malmö and Lund and at Helsingborg Hospital.
  • zone I stage 1 or 2 ROP without plus disease, posterior zone II stage 2 ROP without plus disease, or zone II stage 3 ROP without plus disease. ROP needs to be documented by digital widefield photography and classification confirmed by two ophthalmologist.
Exclusion Criteria
  • ocular infection
  • systemic steroid treatment within two weeks before the start of drop treatment

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Dexamethasone treated infantsDexamethasone Ophthalmic15 infants that receive dexamethasone eye drops for treatment of retinopathy of prematurity will be included, and both serum and saliva samples will be collected in order to find out the pharmacokinetic properties of dexamethasone in eye drops according to a pre-specified sampling scheme specifically designed for this purpose by experts in pediatric pharmacokinetics.
Primary Outcome Measures
NameTimeMethod
Pharmacokinetics: half-life of plasma concentrations of dexamethasone during treatment with dexamethasone eye drops measured with mass spectrometry.up to 14 weeks

Half-life of dexamethasone- t½, hours

Pharmacokinetics: maximum plasma concentration of dexamethasone during treatment with dexamethasone eye drops measured with mass spectrometry.up to 14 weeks

Maximum plasma concentration- Cmax, nmol/L

Pharmacokinetics: saliva koncentrations of dexamethasone during treatment with dexamethasone eye drops.up to 14 weeks

Half-life of dexamethasone- t½, hours

Pharmacokinetics: area under the concentration-time curve for saliva dexamethasone from time point 0 to time t of the last measured concentration above the limit of quantification time.up to 14 weeks

AUC0-t, nmol.h/L

Pharmacokinetics: area under the concentration-time curve for saliva dexamethasone from time point 0 to infinity;up to 14 weeks

AUC0-∞, nmol.h/L

Pharmacokinetics: apparent volume of distributionup to 14 weeks

Vz/F, L

Safety: serum concentrations of endogenous corticosteroids before, during and after treatment with dexamethasone eye drops measured with mass spectrometry.up to 14 weeks

Endogenous levels of corticosteroids, nmol/L

Safety: saliva concentrations of endogenous corticosteroids before, during and after treatment with dexamethasone eye drops.up to 14 weeks

Endogenous levels of corticosteroids, nmol/L

Pharmacokinetics: saliva koncentrations of dexamethasone during treatment with dexamethasone eye drops measured with mass spectrometry.up to 14 weeks

Maximum saliva concentration- Cmax, nmol/L

Pharmacokinetics: time to reach maximum plasma concentration of dexamethasone during treatment with dexamethasone eye drops measured with mass spectrometry.up to 14 weeks

tmax, hours

Pharmacokinetics: time to reach maximum saliva concentrations of dexamethasone during treatment with dexamethasone eye drops measured with mass spectrometry.up to 14 weeks

tmax, hours

Pharmacokinetics: area under the concentration-time curve for plasma dexamethasone from time point 0 to time t of the last measured concentration above the limit of quantification time.up to 14 weeks

AUC0-t, nmol.h/L

Pharmacokinetics: area under the concentration-time curve for plasma dexamethasone from time point 0 to infinity;up to 14 weeks

AUC0-∞, nmol.h/L

Pharmacokinetics: apparent total body clearanceup to 14 weeks

CL/F, L/h

Secondary Outcome Measures
NameTimeMethod
To describe if dexamethasone eye drops delay the intervention for type 1 ROP in cases without regression by calculating the time from detection of type 2 ROP to type 1 ROPUp to 14 weeks

Time from detection of type 2 ROP to type 1 ROP, days

To describe if dexamethasone eye drop treatment before intervention for type 1 ROP reduces the number of recurrences after the intervention.Up to 14 weeks

recurrences after laser/anti-VEGF treatment, percentage

To find out if retinal morphology measured with optical coherence tomography is affected by dexamethasone eye drops at 2.5 years of age.after 2.5 years

Retinal thickness, micrometers

To find out if dexamethasone eye drops affect refractive errors at 2.5 years of ageafter 2.5 years

Refractive power, diopters

To find out if dexamethasone eye drops affect visual acuity at 5 years of ageafter 5 years

Visual acuity according to Snellen, fraction

To find out if dexamethasone eye drops affect refractive errors at 5 years of ageafter 5 years

refractive power, diopters

To find out if retinal morphology measured with optical coherence tomography is affected by dexamethasone eye drops at 5 years of age.after 5 years

Retinal thickness, micrometers

To find out if dexamethasone eye drops affect visual acuity at 2.5 years of ageafter 2.5 years

Visual acuity according to Snellen, fraction

Trial Locations

Locations (2)

Skåne University Hospital

🇸🇪

Lund, Skåne, Sweden

Sahlgrenska University Hospital

🇸🇪

Göteborg, Västra Götaland, Sweden

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