Effect of Embryo Biopsy Side on Post Biopsy Blastocyst Structurs and Implantation
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Preimplantation Diagnosis
- Sponsor
- Ahmad Mustafa Mohamed Metwalley
- Enrollment
- 100
- Locations
- 2
- Primary Endpoint
- blastocyst location of ICM at day 5
- Status
- Completed
- Last Updated
- 7 years ago
Overview
Brief Summary
Selection of side of embryo biopsy that will not interfering with implantation power of developed blastocyst embryo during biopsy procedure arranged for PGT.
Detailed Description
During process of embryo biopsy, it was indicated to avoid contacting cells closer to inner cell mass as it can decrease the quality of embryo and its structural composition. However, recommendation for collection from the trophectoderm (TE) side is indicated. Absence of differentiation during cleavage-stage or morula stage at day 3 and day 4 respectively, will present the needful to focus a specific location for embryo biopsy during pre-blastocyst stages for embryo going to do genetic testing before implantation or embryo transfer. In articulation around the second polar body or the animal pole of embryo, collection of the cell from the vegetal pole in crospondence to avoide collection form the animal pole is to be compare the results of implantation for biopsy done using the animal pole side or the random non organized biosy procedure.
Investigators
Ahmad Mustafa Mohamed Metwalley
IVF laboratory manager
Al Baraka Fertility Hospital
Eligibility Criteria
Inclusion Criteria
- •grade 1 or 2 embryos at eight cell stage or 8-1/ 8-2 embryo quality.
Exclusion Criteria
- •grade 3 embryo at day 3, or 8-3 embryos.
Outcomes
Primary Outcomes
blastocyst location of ICM at day 5
Time Frame: 2 months
describing the location of the inner cell mass (ICM) in location to the zona peluceda (ZP) drilling opening.
Secondary Outcomes
- Implantation power(2 months)