Evaluation of GADD34 and Type I IFN Response Gene Expression in Patients Suffering From Systemic Lupus Erythematosus
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 90
- 主要终点
- GADD34 expression in SLE patients
研究概览
简要总结
GADD34 (Growth Arrest and DNA Damage inducible-protein) is a regulatory subunit of PP1 (phosphatase 1) phosphatase which dephosphorylates eIF2alpha (eucaryotic initiation factor 2 alpha subunit), representing a negative feedback loop of the unfolded protein response (UPR). Moreover, GADD34 is necessary for type I interferon (IFN) production in response to viral infection in murine models. We investigate here the expression of GADD34 in systemic lupus erythematosus (SLE), in which type I IFN has an important pathogenic role.
We report a case-control study on GADD34 gene expression in PBMC (peripheral blood mononuclear cells) of SLE patients (n=60) and age- and sex-matched healthy controls (n=30). The level of GADD34 gene expression, as well as of type-I IFN response genes in PBMC is measured by quantitative PCR.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Man and woman over 18 years old.
- •Patients suffering from SLE (SLICC criteria)
- •Patients having given their "Non-opposition"
- •Patients with social security affiliation (required in France)
排除标准
- •Patients suffering from other auto-immune disease (except SAPL, thyroiditis, and Gougerot Sjogren syndrome)
- •Patients with viral infection within 15 days
- •People with special protection (defined in articles L1121-§5-8 and articles L3212-§1-3 of French health care law)
研究组 & 干预措施
Patients
Patients with SLE
干预措施: Patients
Healthy subjects
干预措施: Healthy subjects
结局指标
主要结局
GADD34 expression in SLE patients
时间窗: 4 years
Evaluation of GADD34 expression in PBMC of patients suffering from SLE compared to GADD34 expression in PBMC of healthy subjets. GADD34 gene expression level is quantified in the PBMC by quantitative PCR
次要结局
- GADD34 and IFN response gene expression(4 years)
- CHOP and BiP expression(4 years)
- GADD34 expression and pathology activity(4 years)