The Anabolic and Catabolic Effect of Loaded and Unloaded Exercise on Articular Cartilage Health

Not Applicable

Completed

• Conditions: Healthy
• Interventions: Other: +12% Body Mass; Other: Control Condition; Other: -12% Body Mass

• Registration Number: NCT05925244
• Lead Sponsor: University of Manitoba

Brief Summary

The serum concentration of cartilage oligomeric matrix protein (sCOMP) has been considered a mechano-sensitive biomarker of articular cartilage turnover, and N-propeptide of type II collagen (PIIANP) is a proposed biomarker of type II collagen synthesis. Few studies have investigated both the anabolic and turnover response of articular cartilage as a result of acute changes in body weight mass during exercise. Using a repeated measures cross-over design, fifteen healthy adults (age 18-30 years) performed three, 30-minute bouts of treadmill walking exercise under 3 loading conditions: (1) control (no alteration to body mass); (2) loaded (12% increase in body mass using a weighted vest); and (3) unloaded (12% decrease in body mass using lower body positive pressure). Venous blood was collected before, immediately after, and 15 and 30 minutes after exercise to investigate cartilage turnover (sCOMP) and anabolism (PIIANP).

Detailed Description

Not available

Study Timeline

• Study Start: 2019-09-27
• Primary Completion: 2020-03-01
• Study Completion: 2020-03-01
• Status Verified: 2023-06
• Study First Submitted: 2023-06-21
• Study First Submitted QC: 2023-06-21
• Last Update Submitted: 2023-06-28
• Study First Posted: 2023-06-29
• Last Update Posted: 2023-07-03

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 15

Inclusion Criteria

• Healthy
• 18-30 years old

Exclusion Criteria

• Traumatic injury to the lower extremity in the past year
• Hip, knee, or ankle surgery
• Radiographic evidence of joint degeneration
• Cardiovascular disease
• Ankylosing spondylitis
• Psoriatic arthritis
• Chronic reactive arthritis
• Renal problems requiring peritoneal dialysis or hemodialysis
• Not currently using any prescription or non-prescription medications and/or nutritional supplements

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
+12% Body Mass	+12% Body Mass	The +12% body mass condition did 30 minutes of walking exercise wearing a weighted vest with +12% of their body mass applied.
Control	Control Condition	The control condition did 30 minutes of walking exercise with their own body mass.
-12% Body Mass	-12% Body Mass	The -12% body mass condition did 30 minutes of walking exercise using a lower body positive pressure treadmill with -12% of their body mass applied.

Primary Outcome Measures

Name	Time	Method
N-propeptide of type II collagen (PIIANP)	PIIANP was measured at baseline, immediately post, and 15- and 30-minutes post exercise.	The systemic blood serum concentration of PIIANP was evaluated in all participants under the 3 conditions in a crossover trial.
Serum cartilage oligomeric matrix protein (sCOMP)	sCOMP was measured at baseline, immediately post, and 15- and 30-minutes post exercise.	The systemic blood serum concentration of sCOMP was evaluated in all participants under the 3 conditions in a crossover trial.

Trial Locations

Locations (1)

University of Manitoba; 🇨🇦 Winnipeg, Manitoba, Canada