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Investigation on the bioavailability and metabolism of resveratrol an ingredient of berries in humans

Not Applicable
Conditions
healthy volunteers
Registration Number
DRKS00008788
Lead Sponsor
Max Rubner-InstitutBundesforschungsinstitut für Ernährung und Lebensmittel
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Complete
Sex
All
Target Recruitment
107
Inclusion Criteria

Healthy women and men (18-65 years old), BMI 20 – 30 kg/m2; no intake of antibiotics within the last 3 monts, no intake of laxatives or other drugs which may influence the intestinal system within the last 3 month; test persons which signed the agreement letter for the participation of this study.

Exclusion Criteria

Intake of antibiotics within the last 3 months, intake of laxatives or other drugs which may influence the intestinal system within the last 3 month; participants with serious diseases affecting the resorption of nutrients, digestion, metabolisation or excretion, pregnancy or lactation, participants who have ingested dietary supplements or drugs within the last 3 monts which may effect the parameters investigated in this study; participants which are expected to not behave compliant, participants with known allergies against RES or intolerance of food of which RES can be extracted, participants who are institutionalized due to an administrative order or court order by a court or a governmental order, participants who did not sign the agreement letter for participation of this study

Study & Design

Study Type
interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Determination of the concentration of resveratrol (RES) and its microbial metabolites in urine (48-h- urine collection) and blood samples (of a subgroup of 12 participants) before and after (1.5, 24 and 48 hours) oral uptake of RES to identify the importance of RES microbial metabolites. Another aim of this study is the isolation and characterisation of lunularin-producing bacterial strains isolated from human fecal samples.
Secondary Outcome Measures
NameTimeMethod
Another aim of this study is the investigation, whether a single oral uptake of RES shows acute effects on selective parameters of the immune System (phagocytosis and respiratory burst, cytokine response of ex-vivo mitogen-activated peripheral blood mononuclear cells, cytolytic activity of natural killer cells (using cytometric bead array, CBA )). <br>Moreover, it will be investigated if RES and its metabolites can be detected both from venous blood samples and from dried blood spots with capillary blood.
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