MedPath

Cardiovascular and InflammAging Study

Not Applicable
Recruiting
Conditions
Aging
Overweight or Obesity
Cardiovascular Diseases
Interventions
Dietary Supplement: Juice Plus+ Fruit, Vegetable and Berry blends
Dietary Supplement: Juice Plus+ Omega blend
Registration Number
NCT04763291
Lead Sponsor
Green Beat
Brief Summary

Evidence from previous studies supports a strong relationship between fruit and vegetable consumption and reduced cardiac risk. This could be mediated via improvements on blood pressure, platelet function and vascular reactivity. Certain vitamins and polyphenols found in fruits and vegetables, have antioxidant and anti-inflammatory effects and play a major role on the function of immune cells. Previous studies have also demonstrated the importance of omega-3 fatty acids on humans' health and their positive effects on the cardiovascular system and blood lipids regulation, as well as their involvement on inflammatory response. Nutritional regimens with adequate intake of micronutrients, fruit and vegetables, omega-3 fatty acids, low in sugar and saturated fats, such as the Mediterranean diet or vegetarian diets, can reduce chronic inflammation and oxidative stress and improve cardiovascular risk profile. Considering that the population's fruit and vegetable and omega-3 intakes are below recommendations, whole food-based supplements could provide an accessible form of supplementation to bridge the gap between actual and recommended intakes. This study is aiming to assess whether long-term separate ingestions of an encapsulated juice powder concentrate and a plant-based omega fatty acid supplement, or a combined ingestion of the two, can affect biomarkers of cardiovascular health, low-grade inflammation and indicators of biological aging in older adults.

Detailed Description

Not available

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
112
Inclusion Criteria
  • Post menopausal
  • Non-smokers
  • BMI 18.5 to 40 kg/m2
  • Vegetable intake ≤ 2 servings/ day
  • Intake of fruits ≤ 2 servings /day
  • Adherence to a 6-week washout period for dietary supplements not ingested for specific medical conditions.
Exclusion Criteria
  • Age <55 and >80 years
  • Smokers (or ex-smokers who quit smoking less than 3 years ago)
  • Aversion to stop the intake of multivitamins, multiminerals or omega fatty acid supplements
  • Subjects with histamine intolerance
  • Hypertension, starting with grade 2 according to the classification of the European Society of Hypertension: systolic blood pressure > 160mmHg, diastolic blood pressure >100 mmHg
  • All medication taken for less than 3 months or with changes on the dosage over the last 3 months and medication for any of the conditions listed below.
  • Clinically relevant infectious diseases
  • Diabetes mellitus type I and type II
  • Rheumatic diseases
  • Auto-immune diseases
  • Any stents and coronary artery diseases (CAD)
  • Cancer patients
  • Pregnancy
  • Significant lifestyle changes, e.g. changes in diet or physical activity profile

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Fruit, Vegetable, Berry and Omega (FVBO) groupJuice Plus+ Omega blendParticipants have to ingest an encapsulated juice powder concentrate along with a plant-based fatty acid supplement, otherwise continue their habitual diet and lifestyle.
Fruit, Vegetable, Berry and Omega (FVBO) groupJuice Plus+ Fruit, Vegetable and Berry blendsParticipants have to ingest an encapsulated juice powder concentrate along with a plant-based fatty acid supplement, otherwise continue their habitual diet and lifestyle.
Fruit, Vegetable and Berry (FVB) groupJuice Plus+ Fruit, Vegetable and Berry blendsParticipants have to ingest an encapsulated juice powder concentrate, otherwise continue their habitual diet and lifestyle.
Omega groupJuice Plus+ Omega blendParticipants have to ingest a plant-based fatty acid supplement, otherwise continue their habitual diet and lifestyle.
Primary Outcome Measures
NameTimeMethod
Lipid profile markers12 months

Total cholesterol, HDL, LDL, ApoA1, triglycerides, Omega-3-Index

Concentration changes in cytokines/ cytokine receptors12 months

TNF-α, sTNFR1 and sTNFR2, CCL5 = RANTES, IL-1β, hsCRP, CCL2 = MCP-1, Osteoprotegerin (OPG), IL-5, IL-8

Glucose metabolism12 months

Glucose, insulin, HOMA-IR, HbA1c

Hemostasis markers12 months

Platelet aggregation, thrombelastometry, coagulation (Quick, PT, PTT)

Oxidative stress markers12 months

oxLDL, MDA, carbonyl proteins, (CP), redox state of albumin, homocysteine

Secondary Outcome Measures
NameTimeMethod
Vitamin K metabolism12 months

Osteocalcin, matrix Gla protein (MGP), vitamins K1 and K2 (MK-7)

mitochondrial DNA copy number (mtDNA-CN)12 months

mitochondrial DNA will be amplified and the ratio to genomic DNA will be calculated

Upper respiratory tract symptoms12 months

The number of symptoms experienced and severity of symptoms will be assessed by the Wisconsin upper respiratory symptoms survey (WURSS).

Quality of life Questionnaire12 months

Assessed through the Short Form Survey (SF-36), which consists of 8 scales, namely: physical functioning, physical role functioning, bodily pain, general health, vitality, social functioning, emotional role functioning, mental health. All items are scored on a scale from 0 to 100 and higher scores denote a more favourable health state.

Neurotrophins12 months

BDNF and NGF will be measured by ELISA

Cognitive function12 months

Cognitive function will be assessed by the CFD-index, which takes into account the following 5 dimensions: Attention (alertness, shared attention, processing speed), Verbal long-term memory, Executive functions (spatial working-memory, cognitive flexibility), Expressive language (word-fluency, object designation), Perceptual-motoric functions (visuoconstruction).

Trial Locations

Locations (2)

Otto Loewi Research Center, Section of Physiological Chemistry, Medical University of Graz

🇦🇹

Graz, Austria

Green Beat

🇦🇹

Graz, Austria

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