Expression Profiling of microRNA Following Administration of Dexmedetomidine

Not Applicable

Completed

• Conditions: Coronary Disease
• Interventions: Drug: Dexmedetomidine Injection

• Registration Number: NCT03213743
• Lead Sponsor: First Affiliated Hospital of Kunming Medical University

Brief Summary

The purpose of the study is to find out the differential expression profiling of microRNA before and after adiministration of dexmedetomidine in patients undergoing procedures, and then investigators will do some in vitro studies to validate the functions of the microRNA.

Detailed Description

Background and objective: The use of dexmedetomidine may have benefits on the clinical outcomes of cardiac surgery. MicroRNAs are non-coding RNA sequences that act as regulators of gene expression. Investigators conduct a study to determine the role of microRNAs in cardioprotection mediated by dexmedetomidine.

Methods: In this study 3 patients were selected. The blood sample were taken before administration of dexmedetomidine.Investigators infused loading dose of dexmedetomidine for 10 minutes and maintaining dese of dexmedetomidine for 20 minutes.Thirty minutes after the administration, another blood samples were taken and stored in the liquid nitrogen and both the samples were marked. Those sample were analyzed for differentially expressed microRNA with Exiqon miRNA Array.

Study Timeline

• Study Start: 2017-06-19
• Primary Completion: 2017-06-21
• Study Completion: 2017-06-21
• Status Verified: 2017-07
• Study First Submitted: 2017-07-07
• Study First Submitted QC: 2017-07-10
• Last Update Submitted: 2017-07-10
• Study First Posted: 2017-07-11
• Last Update Posted: 2017-07-11

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 3

Inclusion Criteria

• The patients without arrithmia will undergo procedure.

Exclusion Criteria

• The patients have bradycardia (hear rate is less than 60 beats per minute) or conduction block.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
before dexmedetomidine	Dexmedetomidine Injection	We took blood samples before the administration of dexmedetomidine to determine exression level of microRNA in the subjects.

Primary Outcome Measures

Name	Time	Method
microRNA differential expression	30 minutes	microRNA differential expression exerts cardioprotection effect on patients undergoing procedures.

Trial Locations

Locations (1)

Jinqiao Qian; 🇨🇳 Kunming, Yunnan, China