Expression Profiling of microRNA Following Administration of Dexmedetomidine in Patients Undergoing Procedures
Overview
- Phase
- Not Applicable
- Intervention
- Dexmedetomidine Injection
- Conditions
- Coronary Disease
- Sponsor
- First Affiliated Hospital of Kunming Medical University
- Enrollment
- 3
- Locations
- 1
- Primary Endpoint
- microRNA differential expression
- Status
- Completed
- Last Updated
- 8 years ago
Overview
Brief Summary
The purpose of the study is to find out the differential expression profiling of microRNA before and after adiministration of dexmedetomidine in patients undergoing procedures, and then investigators will do some in vitro studies to validate the functions of the microRNA.
Detailed Description
Background and objective: The use of dexmedetomidine may have benefits on the clinical outcomes of cardiac surgery. MicroRNAs are non-coding RNA sequences that act as regulators of gene expression. Investigators conduct a study to determine the role of microRNAs in cardioprotection mediated by dexmedetomidine. Methods: In this study 3 patients were selected. The blood sample were taken before administration of dexmedetomidine.Investigators infused loading dose of dexmedetomidine for 10 minutes and maintaining dese of dexmedetomidine for 20 minutes.Thirty minutes after the administration, another blood samples were taken and stored in the liquid nitrogen and both the samples were marked. Those sample were analyzed for differentially expressed microRNA with Exiqon miRNA Array.
Investigators
Jinqiao Qian
Professor
First Affiliated Hospital of Kunming Medical University
Eligibility Criteria
Inclusion Criteria
- •The patients without arrithmia will undergo procedure.
Exclusion Criteria
- •The patients have bradycardia (hear rate is less than 60 beats per minute) or conduction block.
Arms & Interventions
before dexmedetomidine
We took blood samples before the administration of dexmedetomidine to determine exression level of microRNA in the subjects.
Intervention: Dexmedetomidine Injection
Outcomes
Primary Outcomes
microRNA differential expression
Time Frame: 30 minutes
microRNA differential expression exerts cardioprotection effect on patients undergoing procedures.