Validation of two stable isotopic labels for determination of albumin synthesis rate
Completed
- Conditions
- liver function / liver failureMetabolic and Endocrine - Other metabolic disordersOral and Gastrointestinal - Other diseases of the mouth, teeth, oesophagus, digestive system including liver and colon
- Registration Number
- ACTRN12614000541606
- Lead Sponsor
- Jan Wernerman
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- All
- Target Recruitment
- 16
Inclusion Criteria
healthy volunteers
Exclusion Criteria
Age < 18 years
Participation in other trial within 2 months
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method albumin synthesis rate<br><br>Albumin fractional synthesis rate is assessed by the flooding technique (Ballmer et al Am J Physiol 1990; 259: E797-803). The incorporation of labeled phenylalanine in plasma albumin is assessed by gas chromatography-mass spectrometry after separating albumin from other plasma proteins. The precursor pool is assessed from the ratio of labeled to unlabeled phenylalanine in plasma. [In the morning after start of infusion on each study occation. Volunteers will be studied twice (protocol 1) on day 1 and 3, or thrice on day 1, after 5 hrs and after 7 days (protocol 2).]
- Secondary Outcome Measures
Name Time Method Fibrinogen synthesis rate<br><br>Fibrinogen fractional synthesis rate is assessed by the flooding technique (Ballmer et al Am J Physiol 1990; 259: E797-803). The incorporation of labeled phenylalanine in plasma fibrinogen is assessed by gas chromatography-mass spectrometry after separating fibrinogen from other plasma proteins. The precursor pool is assessed from the ratio of labeled to unlabeled phenylalanine in plasma. [In the morning after start of infusion on each study occation. Volunteers will be studied twice (protocol 1) on day 1 and 3, or thrice on day 1, after 5 hrs and after 7 days (protocol 2).]