The Changes of Genes, Proteins, and Metabolites in Patients with Drug-resistant Epilepsy
试验速览
- 阶段
- 不适用
- 状态
- 尚未招募
- 入组人数
- 16
- 试验地点
- 1
- 主要终点
- Single cell RNA sequencing
研究概览
简要总结
In patients with drug-resistant epilepsy (DRE), there may be changes at the genetic, proteomic, and metabolomic levels when comparing epileptic tissues from DRE to normal tissues in traumatic brain injury (TBI). These changes could help in understanding the pathophysiological mechanisms of epilepsy and in identifying new therapeutic targets.
详细描述
Genomical studies have identified changes in the expression of certain genes within epileptic tissues. These genes may be involved in pathways related to the balance of neuronal excitability and inhibition, synaptic transmission, and cell apoptosis.
Proteomic studies will reveal changes in the abundance and modifications of proteins in epileptic tissues. These could involve proteins related to the control of neuronal excitability and synaptic transmission, such as ion channels, neurotransmitter receptors, and synaptic proteins.
Metabolomic researches will reveal changes in metabolites within epileptic tissues. Epilepsy may lead to disruptions in metabolic pathways, affecting key processes such as energy metabolism, amino acid metabolism, and lipid metabolism.
Sample Size: There is no minimum or maximum, but is expected to be far less than 10.
In summary, patients with drug-resistant epilepsy might have changes in genes, proteomics, and metabolomics within epileptic tissues compared to normal tissue from TBI. Further research into these changes will deepen our understanding of the pathophysiology of epilepsy and guide the need for new treatment strategies.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Prospective
入排标准
- 年龄范围
- 14 Years 至 60 Years（Child, Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •14-60 years old, male or female, Han Chinese;
- •Drug-resistant epilepsy;
- •Required surgical implantation of SEEG electrodes.
排除标准
- •Progressive encephalopathy or progressive structural damage in the central nervous system;
- •Significant heart, liver, renal insufficiency, and other medical diseases;
- •Severe side effects from taking antiepileptic drugs at the time of enrollment and not inappropriate for SEEG;
- •Significant intellectual disability;
- •A history of alcohol and drug abuse;
- •Any contraindication to MRI.
结局指标
主要结局
Single cell RNA sequencing
时间窗: through study completion, an average of one year
Cancerous and paracancerous tissues of patients will be subjected to10x Genomics single-cell RNA sequencing, Bulk RNA-seq and spatial transcriptome.
次要结局
- Differentially expressed proteins(through study completion, an average of one year)
- The concentration of metabolites(through study completion, an average of one year)