Biocollection for the Study of Genetic and Immunological Abnormalities in Rare Pediatric-onset Autoimmune and Auto Inflammatory Diseases
Overview
- Phase
- N/A
- Intervention
- Not specified
- Conditions
- Systemic Lupus
- Sponsor
- Hospices Civils de Lyon
- Enrollment
- 400
- Locations
- 13
- Primary Endpoint
- To Identify germline and somatic mutations responsible for rare autoimmune diseases or auto-inflammatory pathologies (pediatric or syndromic or familial) that began in childhood
- Status
- Recruiting
- Last Updated
- 4 months ago
Overview
Brief Summary
Rare diseases are defined as those that affect one person in 2,000, or around three million people in France. The majority of rare diseases are caused by genetics and tend to be severe when they begin in childhood. Autoimmune and autoinflammatory diseases, such as systemic lupus, juvenile dermatomyositis, and juvenile idiopathic arthritis, are examples of rare pediatric diseases. While autoimmune diseases are characterized by an inappropriate adaptive immune response, autoinflammatory diseases involve an excess of the innate immune response. The precise mechanisms of these diseases are not yet fully understood, but recent research has led to advances in their diagnosis and identification, particularly in early onset and familial forms. However, the rarity of these diseases and limited availability of biological samples pose significant challenges.
This study aims to create a biological collection, which includes primary cells (PBMC), DNA, RNA, lymphoblastic lines, and serum, that will help identify genetic and immunological abnormalities in rare autoimmune and autoinflammatory diseases through various research projects.
Detailed Description
A disease is said to be "rare" when it affects one person in 2,000, which represents three million people in France. Most rare diseases (80%) are genetic in origin ; the earlier they start in childhood, the more severe they can be. Rare pediatric diseases include autoimmune diseases (systemic lupus, juvenile dermatomyositis and juvenile idiopathic arthritis) and autoimmune diseases (interferonopathies, FMF, CAPS, TRAPS, and DADA2). Systemic autoimmune diseases are characterized by an inappropriate adaptive immune response (mediated by autoreactive T and/or B lymphocytes) with the production of autoantibodies directed against the constituents of the self (tolerance breakdown). Autoinflammatory diseases, unlike autoimmune diseases, correspond to an excess in the innate immune response (cytokines, macrophages, NK cells, granulocytes, etc.)..The precise pathophysiological mechanisms of these diseases have yet to be fully elucidated. Recent research has led to advances in the diagnosis and identification of monogenic forms of these diseases, particularly in early onset, familial, and syndromic forms. Nevertheless, the rarity of these diseases and limited availability of biological samples are major challenges that need to be overcome. Thus, the aims of this study were as follows: \- The creation of a biological collection: primary cells (PBMC), DNA, RNA, lymphoblastic lines, and serum, which, through various research projects, will help identify genetic and immunological abnormalities in rare autoimmune and autoinflammatory diseases.
Investigators
Eligibility Criteria
Inclusion Criteria
- •minor or adult patient of any age with a rare dysimmune disease characterized by autoimmunity or auto-inflammation or early lymphoproliferation, having started in childhood (\<18 years), or syndromic or familial
- •relative of a minor or adult patient with a rare dysimmune disease characterized by autoimmunity or auto-inflammation or early lymphoproliferation, having started in childhood (\<18 years of age) or syndromic or familial,
- •weight greater than 5 kg
- •Patient/parents/guardians who were informed of the study and signed the consent form.
- •patient affiliated to a social security scheme
- •Healthy volunteer participants
- •minor or adult participants with no age restrictions
- •weight over 5 kg
- •Subject /Parents/guardians who were informed of the study and signed a consent form.
- •Patient affiliated to a social security scheme
Exclusion Criteria
- •\- Subjects /Parents/guardians, refusing to participate in the study
- •Healthy volunteer participants :
- •active infection (viral, bacterial, parasitic)
- •history of neoplasia (\< 5 years) or current neoplasia
- •participants with a personal or family history of autoimmune disease
- •immunocompromised participant (immune deficiency or transplant recipient)
- •Subjects/parents/guardians refusing to participate in the study
- •Adults under legal protection (guardianship, curatorship)
Outcomes
Primary Outcomes
To Identify germline and somatic mutations responsible for rare autoimmune diseases or auto-inflammatory pathologies (pediatric or syndromic or familial) that began in childhood
Time Frame: Baseline
Identification of germline or somatic genetic mutations, based on high-throughput sequencing data (exome, genome or transcriptome).
Secondary Outcomes
- Levels of anti-double stranded DNA(Baseline)
- Levels of complement components C3 and C4(Baseline)
- Level of IFN Signature score(Baseline)
- Concentration of circulating IFN-alpha(Baseline)
- Measurement of disease activity according to Systemic Lupus Erythematosus Disease Activity Index (SLEDAI)(Baseline)
- Presence or absence of anti-type I interferons autoantibodies(Baseline)