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PK Study of Dapagliflozin in Pediatric Subjects With T2DM

Phase 1
Completed
Conditions
Type 2 Diabetes Mellitus
Interventions
Registration Number
NCT01525238
Lead Sponsor
AstraZeneca
Brief Summary

The primary purpose of this study is to evaluate the pharmacokinetics (PK) of Dapagliflozin in pediatric subjects with type 2 diabetes mellitus (T2DM)

Detailed Description

Primary purpose: The primary purpose is to assess the pharmacokinetics of a single dose of Dapagliflozin in the range of 2.5 to 10 mg in pediatric subjects aged 10 to 17 years with T2DM

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
53
Inclusion Criteria
  • Clinical diagnosis of T2DM
  • Male and female subjects ages 10-17
  • Glycosylated Hemoglobin A1c (HbA1c) ≥6 to 10%
  • Body weight ≥30 kg
Exclusion Criteria
  • Fasting plasma glucose (FPG) >240 mg/dL at screening
  • Abnormal renal function
  • Active liver disease and/or significant abnormal liver function

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Dapagliflozin 10 mgDapagliflozin-
Dapagliflozin 2.5 mgDapagliflozin-
Dapagliflozin 5 mgDapagliflozin-
Primary Outcome Measures
NameTimeMethod
Geometric Mean of Maximum Observed Plasma Concentration (Cmax) of Dapagliflozin11 time points: Immediately pre-dose, 0.5, 0.75, 1.0, 1.5, 4, 8, 12, 14, 24, and 48 hours post-dose

Maximum observed plasma concentration (Cmax) was measured by plasma concentration of Dapagliflozin over time. The geometric means are reported in nanograms per milliliter (ng/mL).

Median Time of Maximum Observed Plasma Concentration (Tmax) of Dapagliflozin11 time points: Immediately pre-dose, 0.5, 0.75, 1.0, 1.5, 4, 8, 12, 14, 24, and 48 hours post-dose

Time of maximum observed plasma concentration (Tmax) for Dapagliflozin was derived from plasma concentrations versus time data. Medians were reported in hours (h).

Geometric Mean of Area Under the Concentration-time Curve From Time Zero to the Time of the Last Quantifiable Concentration [AUC(0-T)] of Dapagliflozin11 time points: Immediately pre-dose, 0.5, 0.75, 1.0, 1.5, 4, 8, 12, 14, 24, and 48 hours post-dose

Area under the concentration-time curve from time zero to time of the last quantifiable concentration (AUC(0-T)) was measured by plasma concentration of Dapagliflozin over time. The geometric means are reported in nanogram hours per milliliter (ng\*h/mL).

Mean Plasma Half-life (T-HALF) of Dapagliflozin11 time points: Immediately pre-dose, 0.5, 0.75, 1.0, 1.5, 4, 8, 12, 14, 24, and 48 hours post-dose

Plasma half-life (T-Half) for Dapagliflozin was derived from plasma concentrations versus time data. Means are reported in hours.

Geometric Mean of Apparent Clearance After Extravascular Administration (CL/F) of Dapagliflozin11 time points: Immediately pre-dose, 0.5, 0.75, 1.0, 1.5, 4, 8, 12, 14, 24, and 48 hours post-dose

Apparent clearance after extravascular administration (CL/F) of Dapagliflozin was derived from plasma concentrations versus time data. Geometric means are reported in milliliters per minute (mL/min).

Geometric Mean of Area Under the Plasma Concentration-time Curve From Time Zero Extrapolated to Infinite Time [AUC(INF)] of Dapagliflozin11 time points: Immediately pre-dose, 0.5, 0.75, 1.0, 1.5, 4, 8, 12, 14, 24, and 48 hours post-dose

Area under the plasma concentration-time curve from time zero extrapolated to infinite time was derived from concentration versus time data. Geometric means are reported in nanogram hours per milliliter (ng\*hr/mL).

Geometric Mean of Apparent Volume of Distribution at Terminal Phase After Extravascular Administration (Vz/F) of Dapagliflozin11 time points: Immediately pre-dose, 0.5, 0.75, 1.0, 1.5, 4, 8, 12, 14, 24, and 48 hours post-dose

Geometric mean of apparent volume of distribution at terminal phase after extravascular administration of Dapagliflozin was derived from plasma concentration versus time data. Geometric means are reported in Liters (L)

Secondary Outcome Measures
NameTimeMethod
Geometric Mean of Maximum Observed Plasma Concentration (Cmax) of Dapagliflozin 3-O-Glucuronide11 time points: Immediately pre-dose, 0.5, 0.75, 1.0, 1.5, 4, 8, 12, 14, 24, and 48 hours post-dose

Maximum observed plasma concentration (Cmax) was measured by plasma concentration of Dapagliflozin 3-O-Glucuronide over time. The geometric means are reported in nanograms per milliliter (ng/mL).

Median Time of Maximum Observed Plasma Concentration (Tmax) of Dapagliflozin 3-O-Glucuronide11 time points: Immediately pre-dose, 0.5, 0.75, 1.0, 1.5, 4, 8, 12, 14, 24, and 48 hours post-dose

Time of maximum observed plasma concentration (Tmax) for Dapagliflozin 3-O-Glucuronide was derived from plasma concentrations versus time data. Medians were reported in hours (h).

Geometric Mean of Area Under the Concentration-time Curve From Time Zero to the Time of the Last Quantifiable Concentration [AUC(0-T)] of Dapagliflozin 3-O-Glucuronide11 time points: Immediately pre-dose, 0.5, 0.75, 1.0, 1.5, 4, 8, 12, 14, 24, and 48 hours post-dose

Area under the concentration-time curve from time zero to time of the last quantifiable concentration (AUC(0-T)) was measured by plasma concentration of Dapagliflozin 3-O-Glucuronide over time. The geometric means are reported in nanogram hours per milliliter (ng\*h/mL).

Geometric Mean of Area Under the Plasma Concentration-time Curve From Time Zero Extrapolated to Infinite Time [AUC(INF)] of Dapagliflozin 3-O-Glucuronide11 time points: Immediately pre-dose, 0.5, 0.75, 1.0, 1.5, 4, 8, 12, 14, 24, and 48 hours post-dose

Area under the plasma concentration-time curve from time zero extrapolated to infinite time was derived from concentration versus time data. Geometric means are reported in nanogram hours per milliliter (ng\*hr/mL).

Mean Fasting Plasma Glucose Concentrations at Pre-dose on Day 1 and on Day 2 After an 8-hr FastingDay 1 (Pre-dose) to Day 2

Plasma glucose concentrations were evaluated in all treated subjects at Day 1 pre-dose and at Day 2 after fasting for 8 hours. Means are reported in milligrams per deciliter (mg/dL).

Mean Change in Fasting Plasma Glucose From Baseline Until Day 2Day 1 (Pre-dose) to Day 2

Plasma glucose concentrations were evaluated in all treated subjects at Day 1 pre-dose and at Day 2 after fasting for 8 hours. Mean change from baseline to Day 2 is reported in milligrams per deciliter (mg/dL).

Number of Participants With Marked Abnormalities in Other Chemistry TestingDay 1 (Pre-dose) to Day 3

LLN=Lower Limit of Normal, ULN=Upper Limit of Normal, Pre-Rx=Value before first dose. Lab values that met the following criteria were marked as abnormalities: Glucose, fasting serum (mmol/L): \<0.8\*LLN, \>1.3\*ULN (if Pre-Rx\<LLN: \<0.8\*Pre-Rx, \>ULN. If Pre-Rx\>ULN: \>2.0\*Pre-Rx, \<LLN). Protein (grams per deciliter: g/L): \<0.9\*LLN, \>1.1\*ULN (if Pre-Rx\<LLN: \<0.9\*Pre-Rx, \>ULN. If Pre-Rx\>ULN: \>1.1\*Pre-Rx, \<LLN). Albumin (g/L): \<0.9\*LLN (if Pre-Rx\<LLN: \<0.9\*Pre-Rx). Uric Acid (mmol/L): \>1.2\*ULN (if Pre-Rx\>ULN: \>1.25\*Pre-Rx). Lactate Dehydrogenase (U/L): \>1.25\*ULN (if Pre-Rx\>ULN: \>1.5\*Pre-Rx)

Number of Participants With Marked Urinalysis AbnormalitiesDay 1 (Pre-dose) to Day 3

LLN=Lower Limit of Normal, ULN=Upper Limit of Normal, Pre-Rx=Value before first dose. Lab values that met the following criteria were marked as abnormalities: Blood, urine (Qualitative): \>=2 (If Pre-Rx \>= 1, \>=2\*Pre-Rx). Glucose, urine (Qualitative): \>=1, (If Pre-Rx \>=1, \>=2\*Pre-Rx). Protein, urine (Qualitative): \>=2 (If Pre-Rx \>=1, \>=2\*Pre-Rx). Red Blood Cells (RBC), urine (RBC per High Power Field (hpf)): \>=2 (If Pre-Rx\>=2, \>=4). White Blood Cells (WBC), urine (hpf): \>=2 (If Pre-Rx\>=2, \>=4).

Mean Plasma Half-life (T-HALF) of Dapagliflozin 3-O-Glucuronide11 time points: Immediately pre-dose, 0.5, 0.75, 1.0, 1.5, 4, 8, 12, 14, 24, and 48 hours post-dose

Plasma half-life (T-Half) for Dapagliflozin was derived from plasma concentration versus time data. Means are reported in hours.

Mean Total Amount of Glucose Excreted in Urine Over 24 HoursTime of dose to 24 hours post-dose, Day 1 to Day 2

The total amount of glucose excreted in urine was measured for 24 hours following administration of Dapagliflozin. Means are reported in grams.

Number of Participants With Vital Sign Abnormalities, Electrocardiogram (ECG) Abnormalities, or Physical Examination Abnormalities Following Study Drug Administration.Day 1 to Day 3

Participants were followed from dosing on Day 1 until study discharge on Day 3. The number of participants with investigator-assessed clinically-important abnormalities in vital sign measurements, ECGs or physical examinations was reported.

Number of Participants With Marked Hematology Laboratory AbnormalitiesDay 1 (Pre-dose) to Day 3

LLN=Lower Limit of Normal, ULN=Upper Limit of Normal, Pre-Rx=Value before first dose (Day -1). Lab values that met the following criteria were marked as abnormalities: Hemoglobin (grams per deciliter:g/dL): \<0.85\*Pre-Rx. Hematocrit (%): \<0.85\*Pre-Rx. Platelet Count (x10\^9 cells per liter:c/L): \<0.85\*LLN or \>1.5\*ULN (if Pre-Rx\<LLN, use \<0.85\*Pre-Rx). Leukocytes (x10\^3 cells per microliter: c/uL): \<0.9\*LLN, \>1.2\*ULN (if Pre-Rx\<LLN, use \<0.85\*Pre-Rx or \>ULN, if Pre- Rx\>ULN, use \>1.15\*Pre-Rx or \<LLN). Neutrophils (Absolute) (x10\^3 c/uL): \<=1.5. Lymphocytes (Absolute) (x10\^3 c/uL): \<0.75 or \>7.5. Monocytes (Absolute) (x10\^3 c/uL): \>2.000. Basophils (x10\^3 c/uL): \>0.4. Eosinophils (Absolute) (x10\^3 c/uL): \>0.75. Blasts (Absolute) (x10\^9 c/L) \> 0.

Number of Participants With Marked Serum Chemistry AbnormalitiesDay 1 (Pre-dose) to Day 3

LLN=Lower Limit of Normal, ULN=Upper Limit of Normal, Pre-Rx=Value before first dose. Lab values that met the following criteria were marked as abnormalities: Alkaline Phosphatase (units per liter: U/L), Aspartate Aminotransferase (U/L), Alanine Aminotransferase (U/L): \>1.25\*ULN (if Pre-Rx\>ULN, use \>1.25\*Pre-Rx). Bilirubin (milligrams per deciliter: mg/dL): \>1.1\*ULN (if Pre-Rx\>ULN, use \>1.25\*Pre-Rx). Blood Urea Nitrogen (mg/dL): \>1.1\*ULN (if Pre-Rx\>ULN, use \>1.2\*Pre-Rx). Creatinine (micromoles per Liter (umol/L)): \>1.5\*ULN if Pre-Rx missing or \<= ULN, \>1.33\*Pre-Rx if PreRx \> ULN. Sodium (mmol/L): \>1.05\*ULN, 1.05\*Pre-Rx if Pre-Rx\>ULN: \<0.95\*Pre-Rx, \>ULN. If Pre-Rx\>ULN: \>1.05\*Pre-Rx, \<LLN). Potassium(mmol/L), Chloride (mmol/L), Calcium(mmol/L): \<0.9\*LLN, \>1.1\*ULN (if Pre-Rx\<LLN: \<0.9\*Pre-Rx, \>ULN. If Pre-Rx\>ULN: \>1.1\*Pre-Rx, \<LLN). Phosphorus (mg/dL): \<0.85\*LLN, \>1.25\*ULN (if Pre-Rx\<LLN, \<0.85\*Pre-Rx, \>ULN. if Pre-Rx\>ULN: \>1.25\*Pre-Rx, \<LLN).

Trial Locations

Locations (15)

Emory University

🇺🇸

Atlanta, Georgia, United States

Lsuhsc-Shreveport

🇺🇸

Shreveport, Louisiana, United States

Children'S Hospital Of Philadelphia

🇺🇸

Philadelphia, Pennsylvania, United States

Children'S Hospital Of Pittsburgh Of Upmc

🇺🇸

Pittsburgh, Pennsylvania, United States

Childrens Mercy Hospital

🇺🇸

Kansas City, Missouri, United States

Mercy Children'S Hospital

🇺🇸

Toledo, Ohio, United States

Kosair Charities Pediatric Clinical Research Unit

🇺🇸

Louisville, Kentucky, United States

Christus Santa Rosa Childrens Hospital

🇺🇸

San Antonio, Texas, United States

Nemours Childrens Hospital

🇺🇸

Orlando, Florida, United States

Promedica Toledo Children'S Hospital

🇺🇸

Toledo, Ohio, United States

Women And Children'S Hopsital Of Buffalo

🇺🇸

Buffalo, New York, United States

Local Institution

🇲🇽

Veracruz, Mexico

Axis Clinical Trials

🇺🇸

Los Angeles, California, United States

The Children Hospital Of Alabama

🇺🇸

Birmingham, Alabama, United States

Methodist Le Bonheur Hlthcare

🇺🇸

Memphis, Tennessee, United States

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