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Alteplase-Tenecteplase Trial Evaluation for Stroke Thrombolysis- (ATTEST)

Phase 2
Completed
Conditions
Stroke
Interventions
Registration Number
NCT01472926
Lead Sponsor
NHS Greater Glasgow and Clyde
Brief Summary

A pilot evaluation of tenecteplase compared to alteplase in acute ischaemic stroke patients currently eligible for intravenous alteplase treatment in a prospective, randomised, blinded outcome evaluation clinical trial using brain imaging as a biomarker.

Detailed Description

Newer thrombolytic agents such as tenecteplase have pharmacological features (higher fibrin binding specificity and longer half-life) that may be advantageous when compared to older agents such as alteplase with respect to arterial recanalisation, ease of administration, and reduced bleeding risk. No other clinical trial is currently evaluating alternative thrombolytic strategies in patients who are eligible to receive standard intravenous alteplase, instead concentrating on extending the population for IV thrombolysis.

The ATTEST pilot phase will use brain imaging as a biomarker for key clinical response variables, with penumbral salvage as the primary end-point and secondary end-points including recanalisation as well as conventional clinical scales.

The findings of this study are anticipated to provide data on sample size and event rates to inform the design of a definitive, confirmatory, pragmatic, randomised, controlled trial with clinical endpoints.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
104
Inclusion Criteria
  • clinical diagnosis of supratentorial acute ischaemic stroke with score of at least 1 on the NIH Stroke Scale
  • male or non pregnant female >=18 years
  • within 4.5 hours of onset as defined by time since last known well
  • CT perfusion and CT Angiogram examination acquired prior to treatment
Exclusion Criteria
  • Contraindications to thrombolytic drug treatment for stroke

    • Evidence of intracranial haemorrhage or significant non-stroke intracranial pathology (including central nervous system neoplasm, aneurysm or arteriovenous malformation) on pre-treatment CT
    • Established hypodensity on pre-treatment brain CT of more than one third of the middle cerebral artery territory or Alberta Stroke Programme Early CT (ASPECT) Score <4 (sulcal effacement or loss of grey-white differentiation in cortical territories alone are not counted towards ASPECT score)
    • Hypodensity consistent with recent cerebral ischaemia other than the presenting event
    • Very severe stroke (eg NIHSS>25)
    • systolic blood pressure (BP)> 185 or diastolic BP> 110 mm Hg, or aggressive management (intravenous pharmacotherapy) necessary to reduce BP to these limits
    • If on warfarin, International Normalised Ratio (INR) <1.4
    • Current prescription of non-warfarin oral anticoagulant drugs
    • Significant abnormality of coagulation parameters pre-treatment (prolonged INR or activated partial thromboplastin time (APTT), or platelet count <100,000/mm3)
    • administration of heparin within the previous 48 hours and a thromboplastin time exceeding the upper limit of normal for laboratory, or use of therapeutic dose low molecular weight heparin within 48h
    • Clinical history suggestive of subarachnoid haemorrhage even if no blood is evident on CT
    • Risk of bleeding (Major surgery within previous 1 month; intracranial or spinal surgery; recent trauma to the head or cranium; prolonged cardiopulmonary resuscitation (> 2 minutes) within the past 2 weeks; acute pericarditis and/or subacute bacterial endocarditis; acute pancreatitis; severe hepatic dysfunction, including hepatic failure, cirrhosis, portal hypertension (oesophageal varices) and active hepatitis; active peptic ulceration; any known history of haemorrhagic stroke or stroke of unknown origin; arterial aneurysm and known arteriovenous malformation)
    • Dependent (mRS 3-5) pre-stroke
    • Blood glucose <2 mmol/l or >18 mmol/l
    • Seizure at onset of symptoms unless brain imaging identifies positive evidence of significant brain ischaemia (eg CTA confirmed arterial occlusion, early ischaemic change on plain CT, hypoperfusion on CTP)
    • Pregnancy
  • Known impaired renal function (estimated Glomerular Filtration Rate <30 ml/min) precluding contrast CT

  • Known allergy to radiological contrast

  • History of allergies to active substances in either trial medication, or to excipients including gentamicin

  • Severe concurrent medical condition that would prevent participation in study procedures (e.g. cardia failure with severe pulmonary oedema)or with life expectancy <=3 months

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Tenecteplase 0.25 mg/kgTenecteplaseIntravenous tenecteplase 0.25 mg/kg (single bolus, maximum 25 mg)
Alteplase 0.9 mg/kgalteplaseIntravenous alteplase 0.9 mg/kg (10% bolus and 90% as IV infusion over 1 hour, maximum 90 mg)
Primary Outcome Measures
NameTimeMethod
Percent penumbral salvage at 24-48h (initial penumbra volume on computed tomography perfusion (CTP) imaging versus 24-48h CT infarct volume.48 hours

Percent penumbral salvage at 24-48h (initial CTP-defined penumbra volume versus 24-48h CT infarct volume.

Secondary Outcome Measures
NameTimeMethod
Proportion of patients with symptomatic intracerebral haemorrhage (SICH) on 24-48 hour CT48 hours

Proportion of patients with symptomatic ICH (SICH) on 24-48 hour CT:

* by Safe Implementation of Thrombolysis Monitoring Study (SITS-MOST) definition - parenchymal haematoma type 2 (PH2/PHr2) + NIHSS deterioration by \>=4 points at 24 hours

* Any ICH

Distribution of functional outcome scores (mRS) at Day 9090 days

Distribution of functional outcome scores (mRS)

Proportion of patients with favourable clinical outcome (mRS 0-1) at Day 9090 days

Proportion of patients with favourable clinical outcome (mRS 0-1)

Distribution of functional outcome by modified Rankin Scale (mRS) scores at Day 3030 Days

Distribution of outcome scores on the modified Rankin Scale (mRS)

Proportion of patients exhibiting recanalisation (on computed tomography angiography, CTA) 24-48 hours post treatment48 hours

Proportion of patients exhibiting recanalisation (measured by CTA) 24-48 hours post treatment

Mortality at Day 9090 Days
Early clinical improvement 24 hours post treatment24 hours

Early clinical improvement (National Institutes of Health Stroke Scale \[NIHSS\] score reduced by \>=4 points, or = 0 or 1) 24 hours post treatment

Proportion of patients with favourable clinical outcome (mRS 0-1) at Day 3030 days

Proportion of patients with favourable clinical outcome (mRS 0-1)

Average 'home time' by day 9090 Days

Average 'home time' (number of nights spent in non-institutional private residence) by Day 90

Trial Locations

Locations (1)

Southern General Hospital

🇬🇧

Glasgow, Scotland, United Kingdom

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