Identification of Target Genes for Diagnosis and Prognosis of AML Using a Custom-Design Microarray
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 96
- 试验地点
- 2
- 主要终点
- Correlation of increased or decreased expression of same transcripts with disease outcome
研究概览
简要总结
RATIONALE: Studying samples of blood and bone marrow in the laboratory from patients with cancer may help doctors learn more about changes that occur in DNA and identify genes related to cancer. It may also help doctors diagnose cancer and predict how patients will respond to treatment.
PURPOSE: This research study is identifying cancer-related genes in blood and/or bone marrow samples from patients with acute myeloid leukemia.
详细描述
OBJECTIVES:
- To identify and validate individual genes for diagnosis of three major translocations in acute myeloid leukemia.
- To correlate transcript expression data in the various translocations with age, sex, race, response to treatment, and survival and with other known mutations.
OUTLINE: Blood and/or bone marrow samples previously procured from patients on CALGB-9665 are obtained from the CALGB Leukemia Tissue Bank from patients enrolled on CALGB AML treatment studies. Mononuclear cells are isolated from samples and mRNA is extracted. Gene expression profiles are analyzed via custom mRNA microarray and confirmed by quantitative real-time PCR.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Retrospective
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- 未提供
排除标准
- 未提供
结局指标
主要结局
Correlation of increased or decreased expression of same transcripts with disease outcome
时间窗: baseline
Minimum number of genes that can be used for precise diagnosis of each of the three subtypes of acute myeloid leukemia
时间窗: baseline
Identification of individual genes that are differentially expressed between the subtypes of AMLs
时间窗: baseline
Correlation of the patterns of expression of the translocation-specific transcripts with age, sex, race, response to treatment, survival, and with other known mutations
时间窗: baseline
次要结局
未报告次要终点