Study of blood clot product following processing of blood in medical grade glass and plastic test tubes

Not Applicable

Not yet recruiting

• Registration Number: CTRI/2020/02/023383
• Lead Sponsor: Dr Sukalkar Shrushti Mahendra

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 24 November 2021

Recruitment & Eligibility

• Status: Not Yet Recruiting
• Sex: Not specified
• Target Recruitment: 0

Inclusion Criteria

1.Systemically healthy participants above the age of 18 years of age rendering informed consent to participate in the study.

2.Normal hemogram with parameters of Hb, WBC,RBC, and platelet counts within normal range.

Hb female: 12-15mg/dl

Hb male: 13-17mg/dl

RBC: 4.1-5.1 million/cu.mm

WBC: 4400-11300 cells/mcl

Platelet counts: 150,000-450,000 micro liter.

Exclusion Criteria

1.History of hospitalization within past 3-6 months.

2.Participants on antibiotics or any drugs past 6 months

3.Participants taking anticoagulant or anti-platelet drugs.

4.Participants with habit of smoking or alcohol intake.

5.Pregnant and lactating mothers.

6.Participants in active phase of menstruation (day 1-7).

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
1.Difference in platelets and WBC counts between the whole blood and residual serum assessed using an automated cell analyzer following centrifugation of 10 ml of blood in either borosilicate glass or polypropylene plastic centrifugation tubes abiding to PRF and A-PRF centrifugation protocols.Timepoint: One time blood collection at baseline.

Secondary Outcome Measures

Name	Time	Method
The distribution of platelets and WBCs within the head, middle and tail region of the fibrin mesh of platelet rich fibrin (PRF) and advanced platelet rich fibrin (A-PRF) membranes generated through centrifugation of 10 ml blood using either borosilicate glass or polypropylene centrifugation test tubes abiding to PRF and A-PRF centrifugation protocols under light microscopy at 40 x magnification. <br/ ><br>Timepoint: one time blood collection at baseline.