Selecting Specific Bio-markers and Researching Mechanisms of Immune Regulation From Inhalation Injury and Traumatic Lung Injury Based on Single Cell Sequencing Technology
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Traumatic Lung Injury
- Sponsor
- Changhai Hospital
- Enrollment
- 21
- Locations
- 1
- Primary Endpoint
- Partial pressure of carbon dioxide in arterial blood, PaCO2
- Status
- Completed
- Last Updated
- 3 years ago
Overview
Brief Summary
The goal of this observational study is to profile the circulating immunological traits of patients with traumatic lung injury (TLI) and inhalation injury (ILI),providing a new direction for the depth research of the pathogenesis, and providing new immune-related targets for diagnosis of the severity of lung injury and treatment.
The investigators performed scRNA-seq of neutrophil and peripheral blood mononuclear cells (PBMCs) from 10 ml fresh circulating blood from 3 patients with TLI and 3 patients with ILI at longitudinal timepoints,as well as peripheral blood from 3 health controls(HCs).
Detailed Description
The investigators performed single-cell transcriptomic sequencing (BD Rhapsody) on neutrophil and PBMCs extracted from the fresh circulating blood from 3 patients with TLI and 3 patients with ILI at longitudinal timepoints,as well as peripheral blood from 3 health controls(HCs). Three time points are picked by clinical conditions of patients with TLI or ILI: day 1 post hospital admission, day 3 post hospital and the day before discharged which matched to acute stage,progressive stage and convalescence.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Lung injury caused by burn trauma is confirmed, and the patient is hospitalized within 24 hours after burn or trauma (the clinician can make a definite diagnosis of lung injury based on the comprehensive judgment of the patient's etiology, history, clinical manifestations, blood gas analysis, laboratory tests and imaging);
- •Patients with inhalation injury caused by head, face and neck burns or inhalation injury combined with multiple body burns \< 30%;
- •Patients with simple inhalation injury;
- •The normal control group should have no smoking history and be normal population;
- •According to the provisions of Good Clinical Practice(GCP), the informed consent has been obtained and the informed consent has been signed to volunteer for the test.
Exclusion Criteria
- •Basic pulmonary diseases like chronic obstructive pulmonary disease (COPD), asthma and et al;
- •Severe organ dysfunction;
- •Autoimmune diseases;
- •Severe infection;
- •Pregnant or nursing women;
- •Fatal or life-threatening events caused by trauma;
- •Any history which may interfere the results or increase the risk of patients judged by researchers.
Outcomes
Primary Outcomes
Partial pressure of carbon dioxide in arterial blood, PaCO2
Time Frame: through study completion, an average of 2 weeks
Computerized tomography, CT
Time Frame: through study completion, an average of 2 weeks
according to the proportion of rib fracture, pneumothorax, hemothorax,lung contusion in the image
Oxygenation index and Fraction of Inspired Oxygen will be combined to report FiO2/PaO2
Time Frame: through study completion, an average of 2 weeks