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Thromboelastographic Methods in Deciding Therapy on Moderate to Severe COVID-19 Patients

Completed
Conditions
COVID-19
Interventions
Diagnostic Test: Standard coagulation profile laboratory examination
Diagnostic Test: Thromboelastography
Registration Number
NCT05343728
Lead Sponsor
Indonesia University
Brief Summary

This study is a prospective observational study that compares the anticoagulant therapy decision time among moderate and severe COVID-19 patients whose coagulation profiles were tested with thromboelastography (TEG) to those with a standard coagulation profile laboratory examination.

Detailed Description

A prospective observational study was conducted among moderate to severe COVID-19 patients in the High Care Unit and Intensive Care Unit at Kiara Ultimate, Cipto Mangunkusumo Hospital, Indonesia. We consecutively recruited 100 moderate and severe COVID-19 patients in the high and intensive care units.

Baseline characteristic data collection was carried out when the patient was admitted to the ICU or HCU. Venous blood was taken by nurses within 30 min of admission. The samples were sent to the centralized hospital laboratory for a standard hemostasis panel which consisted of platelets, PT/APTT, fibrinogen, and D-dimer. The hospital used Sysmex® CS-2500 and 5100 (Sysmex Corp., Japan) for coagulation tests by using plasma derived from centrifuged whole blood samples. Bedside TEG examination was done by using TEG 5000 Hemostasis Analyzer (Haemonetics Corp., USA) with 2.5 ml of a whole blood sample. Samples of patients with heparin thromboprophylaxis were given heparinase, which binds to heparin so that the coagulation profile can be assessed. Except for TEG, all patients received equal diagnostic tests and treatment, including anticoagulant therapy, as indicated according to the national guideline. A trained doctor from the research team recorded time from blood samples taken until the coagulation panel or TEG results were obtained. Test result timestamp, anticoagulation decision timestamp, mortality, and length of stay in higher care were objectively retrieved through online medical records.

We compared the result turnaround time, time to decision of anticoagulant therapy, and clinical outcomes (length of stay and 30-day mortality) between those who had TEG examination in addition to standard coagulation profile examination (thrombocyte count, PT, APTT, D-dimer, and fibrinogen) and those who had a standard coagulation profile laboratory examination only.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
100
Inclusion Criteria
  1. Confirmed COVID-19 (Positive SARS-CoV-2 PCR test), 3. Moderate or severe symptoms (We defined moderate COVID-19 as patients with clinical signs of pneumonia with SpO2 >93% in room air and severe COVID-19 as pneumonia with SpO2 ≤93% in room air or ventilator support requirement), and 4. The family signed the informed consent
Exclusion Criteria
  1. History of blood clotting disorders (eg, hemophilia and Von Willebrand's disease),

  2. Have contraindications to the administration of anticoagulants, for example:

    1. Platelet count < 25,000
    2. Has bleeding manifestations
    3. History of heparin allergy or heparin-induced thrombocytopenia
  3. Pregnant

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Standard coagulation profile laboratory examinationStandard coagulation profile laboratory examinationPatients whose coagulation profile were done by using a standard coagulation profile laboratory examination (thrombocyte count, PT, APTT, D-dimer, Fibrinogen)
Thromboelastographic MethodsStandard coagulation profile laboratory examinationPatients whose coagulation profile were done by using a thromboelastography in addition to standard coagulation profile laboratory examination (thrombocyte count, PT, APTT, D-dimer, Fibrinogen)
Thromboelastographic MethodsThromboelastographyPatients whose coagulation profile were done by using a thromboelastography in addition to standard coagulation profile laboratory examination (thrombocyte count, PT, APTT, D-dimer, Fibrinogen)
Primary Outcome Measures
NameTimeMethod
Time to anticoagulant therapy decisionFirst day of admission in ICU/HCU

Calculation of the time (minutes) required from blood sample was taken until the decision on anticoagulant therapy made by the supervisors

Secondary Outcome Measures
NameTimeMethod
Result turnaround timeFirst day of admission in ICU/HCU

Calculation of the time (minutes) required from blood sample was taken until the examination result was done

Length of stay in higher careDuring hospitalization

Length of stay in intensive care unit and/or high care unit during admission

Mortality30 days after hospital admission

Death within 30 days after admission to the intensive care unit and/or high care unit

Trial Locations

Locations (1)

Cipto Mangunkusumo Cental National Hospital

🇮🇩

Jakarta, DKI Jakarta, Indonesia

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