Effect of Procedural-Related Variables on Melanocyte-Keratinocyte Suspension Transplantation in Non-Segmental Stable Vitiligo: A Clinical and Immunohistochemical Study
Trial Snapshot
- Phase
- Phase 1
- Status
- Completed
- Sponsor
- Cairo University
- Enrollment
- 40
- Primary Endpoint
- The number of patients with satisfactory response after the surgical procedure for vitiligo.
Study Overview
Brief Summary
ORSHFS grafts yielded lower cell counts than NCECS but the M-K ratio and resultant repigmentation did not show significant difference with better healing of the donor site. Compared to cryoblebs, CO2 gave more homogenous pigmentation. The acral sites showed better results using combination of donor NCECS and recipient cryoblebs.
Detailed Description
Background: Melanocyte-keratinocyte suspension (M-K susp) is gaining popularity for vitiligo treatment. Few studies have addressed procedure-related variables.
Objective
To assess the effect of different M-K susp procedure-related variables on the clinical outcome in stable vitiligo.
Methods: This retrospective study included 40 cases with stable non-segmental vitiligo treated by M-K susp followed by narrow band- ultraviolet B phototherapy twice weekly. Donor site was either a skin graft in non-cultured epidermal cell suspension (NCECS) (34 cases) or hair follicle unit in Outer Root Sheath Hair Follicle Suspension (ORSHFS) (6 cases). Recipient site was prepared by either cryoblebbing (24 cases) or carbon dioxide (CO2)laser resurfacing (16 cases). Cell counts and viability were recorded in the cell suspensions, and tissue melanocytes and keratinocytes were examined by Melan A and Cytokeratin respectively. Assessment of repigmentation was done 18 months after the procedure.
Study Design
- Study Type
- Interventional
- Allocation
- Na
- Intervention Model
- Single Group
- Primary Purpose
- Treatment
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to — (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •Stable non-segmental vitiligo
Exclusion Criteria
- •Active vitiligo
Outcomes
Primary Outcomes
The number of patients with satisfactory response after the surgical procedure for vitiligo.
Time Frame: one year
Secondary Outcomes
No secondary outcomes reported
Investigators
Dalia Abdel Halim
Assisstant professorof dermatology
Cairo University
