The Role of Biomarkers in the Occurrence and Development of Asthma
- Conditions
- Asthma in Children
- Interventions
- Diagnostic Test: The level of biomarkers in Peripheral blood
- Registration Number
- NCT05768399
- Lead Sponsor
- Children's Hospital of Chongqing Medical University
- Brief Summary
To study the expression level of biomarkers such as GITRL in peripheral blood of children with asthma and analyze their correlation with the severity of asthma. To study the predictive value of biomarkers level for the occurrence and development of asthma. And to provide supplementary detection means and predictive indicators for the diagnosis of asthma and the severity of asthma.
- Detailed Description
Recruit 30 children with asthma and 30 healthy children based on the consent of the children and their parents or their guardian. Collect and record general information such as name, age, time of first diagnosis, allergens or triggers, daily dosage and frequency of asthma control drugs, home address and contact information. Collect 5 ml of blood sample for the detect of the biomarkers. Asthma severity was assessed using pulmonary function by spirometry and score of C-CAT. Finally study the correlation between the level of biomarkers and asthma severity and the role of biomarkers in the occurrence and development of asthma.
Recruitment & Eligibility
- Status
- RECRUITING
- Sex
- All
- Target Recruitment
- 2
- In line with the diagnostic criteria in the Guidelines for the Diagnosis and Prevention of Bronchial Asthma in Children (2016 Edition)
- Age: 4-12 years old for new diagnosis or recurrent episodes
- Typical clinical manifestations and complete clinical data
- Family members or guardians of children with informed research and signed consent
- All healthy control subjects had no history of lung disease, allergic rhinitis or any other atopic disease
- Suffering from other respiratory diseases (such as bronchiectasis, allergic rhinitis, respiratory failure, tuberculosis, obstructive airway disease) or other inflammatory diseases.
- Abnormal development of congenital airway and lung tissue
- History of immunosuppressant or glucocorticoid use in the first 4 weeks
- Suffering from other endocrine system and immune system diseases
- Incomplete clinical data or examinations.
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Asthma group The level of biomarkers in Peripheral blood Children with asthma diagnosed by a respiratory physician Control group The level of biomarkers in Peripheral blood Children without respiratory disease or inflammatory diseases
- Primary Outcome Measures
Name Time Method The level of biomarkers 2years All participants will be extracted 5ml peripheral blood into the anticoagulant tube by nurses and the peripheral blood will be detected for biomarkers of asthma using RT-qPCR、 ELISA、 Flow cytometry and other methods.
- Secondary Outcome Measures
Name Time Method Pulmonary function（FEV1%） 2 years The correlation of pulmonary function （FEV1） and the level of biomarkers in children with asthma.The lung function test will be performed by professional technicians and the results will be interpreted by specialists.
Pulmonary function（FVC%） 2 years The correlation of pulmonary function （FVC%） and the level of biomarkers in children with asthma.The lung function test will be performed by professional technicians and the results will be interpreted by specialists.
Pulmonary function（FEV1/FVC） 2 years The correlation of pulmonary function （FEV1/FVC） and the level of biomarkers in children with asthma.The lung function test will be performed by professional technicians and the results will be interpreted by specialists.
Pulmonary function（PEF%） 2 years The correlation of pulmonary function （PEF%） and the level of biomarkers in children with asthma.The lung function test will be performed by professional technicians and the results will be interpreted by specialists.
specific IgE 2 years The correlation of blood specific IgE and the level of biomarkers in children with asthma
Childhood Asthma Control Test (C-ACT) 2 years The correlation of the score of C-ACT and the level of biomarkers in children with asthma. Children with asthma and their guardians will be asked for filling the questionnaire of Childhood Asthma Control Test (C-ACT). And then the doctor will check and calculate the scores after completion.
Peripheral blood eosinophil count 2 years The correlation of peripheral blood eosinophil count and the level of biomarkers in children with asthma
Trial Locations
- Locations (1)
Shaoqing Zhang
🇨🇳Chongqing, Chongqing, China