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IL-35+Breg/Il-35 Effect on T Cell Immune in Patients With CHB

Completed
Conditions
Chronic Hepatitis b
Registration Number
NCT03734783
Lead Sponsor
Changhai Hospital
Brief Summary

The investigators observed the level of IL-35 secreting B regulatory (IL-35+Bregs) cells in peripheral blood cells in patients with chronic hepatitis B, and analysed the relationship between the IL-35+Bregs level and disease stage, and Th1 and Th2 cells level.

Detailed Description

Regulatory B cells (Bregs) are involved in the immune tolerance process through multiple pathways and Bregs are involved in the process of chronic HBV infection.

Here the investigators intended to investigate the regulating role and the mechanism of CD19+ IL-35+ cells on host T cell immune during chronic HBV infection by using in vitro cell sorting, B cell differentiation induction, Transwell cell co-culture system and other methods.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
55
Inclusion Criteria
  • Clinical diagnosis of chronic hepatitis B virus infection
  • 17<age<70
  • Can read, understand and sign patients informed consent
Exclusion Criteria
  • Co-infection with HCV,HIV,HDV etc.
  • Have malignancy
  • Have uncontrollable extra-hepatic disease

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
IL-35+Bregs regulatory effectenrolled day

regulatory effect of IL-35+Bregs on T cell immune

IL-35+Bregs levelENROLLED DAY

IL-35 secreting regulatory B cells level in patients with CHB

Secondary Outcome Measures
NameTimeMethod
IL-35+Bregs phenotypeenrolled day

the main phenotype of IL-35 secreting Bregs

Trial Locations

Locations (1)

Changhai hospital

🇨🇳

Shanghai, China

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