Pericardium membrane v/s collagen membrane with PRF in the treatment of furcation defects – a clinico -radiographic study

The study will begin with a non-surgical phase, during which case history will be recorded in a standard proforma after obtaining written consent. Oral hygiene instructions will be provided, followed by scaling and root planing to control supra- and sub-gingival plaque. Clinical parameters, including plaque index (PI), gingival index (GI), probing pocket depth (PPD), clinical attachment level (CAL), and percentage of bleeding sites, along with radiographic parameters using CBCT for three-dimensional visualization and measurement of furcation defects (from cementoenamel junction to alveolar crest), will be recorded at baseline, 3 months, and 6 months after regenerative therapy. PRF will be prepared following Choukroun’s protocol by collecting 10 mL of blood without anticoagulant in a glass or glass-coated tube, immediately centrifuging at 2700 rpm for 12 minutes, and isolating the middle PRF clot between the acellular plasma layer and red corpuscle base. In the surgical phase, after routine preoperative tests, the procedure will be performed under local anesthesia (2% lignocaine with adrenaline 1:200,000). A full-thickness mucoperiosteal flap will be raised, and the furcation defect will be debrided. The defect will be filled with PRF combined with a Type I collagen membrane in Group A and PRF combined with a pericardium membrane in Group B, followed by flap suturing and standard postoperative care.