Fatty Acid Biomarkers of Carbohydrate Intake
Not Applicable
Completed
- Conditions
- Dyslipidemias
- Interventions
- Other: Low carbohydrate dietOther: High carbohydrate, high sugar dietOther: High carbohydrate, low sugar diet
- Registration Number
- NCT03295448
- Lead Sponsor
- Uppsala University
- Brief Summary
The overall aim is to investigate if circulating fatty acids and lipids are influenced by alterations in carbohydrate amount and quality.
- Detailed Description
In this isocaloric clinical cross-over trial, the investigators aim to investigate if circulating fatty acids and lipids are influenced by alterations in carbohydrate amount and quality. The investigators intend to evaluate if changes in carbohydrate quantity and quality (including sugar) influence circulating even-chain saturated fatty acids or if fatty acid-derived indices of desaturation and de novo lipogenesis are more sensitive biomarkers of carbohydrate intake. By performing untargeted lipidomic analyses, the investigators aim to search for novel lipid biomarkers of carbohydrate quantity and quality. The investigators also want to evaluate the influence of moderate alterations of carbohydrate and sugar intake on lipoproteins, insulin sensitivity, inflammatory proteins.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 19
Inclusion Criteria
- BMI 20-38
Exclusion Criteria
- abnormal lab tests (assessed by screening and judged by a physician), diabetes, cardiovascular, kidney, liver or malignant disease, and weight-loss (±3kg) the preceding 3 months of screening. Extreme diets, i.e. very-low carbohydrate diets, intermittent fasting, vegan diets or allergic to gluten
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Fiber - Sugar - Fat Low carbohydrate diet Intervention: Consuming diets rich in fiber, rich in sugar, and rich in fat during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Fat - Fiber - Sugar Low carbohydrate diet Intervention: consuming diets rich in fat, rich in fiber, and rich sugar during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Sugar - Fiber - Fat High carbohydrate, high sugar diet Intervention: Consuming diets rich in sugar, rich in fiber, and rich in fat during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Sugar - Fiber - Fat High carbohydrate, low sugar diet Intervention: Consuming diets rich in sugar, rich in fiber, and rich in fat during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Sugar - Fat - Fiber Low carbohydrate diet Intervention: Consuming diets rich in sugar, rich in fat, and rich in fiber during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Sugar - Fat - Fiber High carbohydrate, high sugar diet Intervention: Consuming diets rich in sugar, rich in fat, and rich in fiber during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Fiber - Fat - Sugar High carbohydrate, high sugar diet Intervention: consuming diets rich in fiber, rich in fat, and rich in fiber during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Fat - Sugar - Fiber High carbohydrate, high sugar diet Intervention: consuming diets rich in fat, rich in sugar, and rich in fibers during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Fat - Sugar - Fiber High carbohydrate, low sugar diet Intervention: consuming diets rich in fat, rich in sugar, and rich in fibers during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Fat - Fiber - Sugar High carbohydrate, low sugar diet Intervention: consuming diets rich in fat, rich in fiber, and rich sugar during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Sugar - Fat - Fiber High carbohydrate, low sugar diet Intervention: Consuming diets rich in sugar, rich in fat, and rich in fiber during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Fat - Sugar - Fiber Low carbohydrate diet Intervention: consuming diets rich in fat, rich in sugar, and rich in fibers during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Fat - Fiber - Sugar High carbohydrate, high sugar diet Intervention: consuming diets rich in fat, rich in fiber, and rich sugar during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Sugar - Fiber - Fat Low carbohydrate diet Intervention: Consuming diets rich in sugar, rich in fiber, and rich in fat during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Fiber - Sugar - Fat High carbohydrate, high sugar diet Intervention: Consuming diets rich in fiber, rich in sugar, and rich in fat during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Fiber - Fat - Sugar Low carbohydrate diet Intervention: consuming diets rich in fiber, rich in fat, and rich in fiber during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Fiber - Sugar - Fat High carbohydrate, low sugar diet Intervention: Consuming diets rich in fiber, rich in sugar, and rich in fat during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet. Fiber - Fat - Sugar High carbohydrate, low sugar diet Intervention: consuming diets rich in fiber, rich in fat, and rich in fiber during treatment periods 1, 2, and 3, respectively. Treatment periods are separated by one week of habitual diet.
- Primary Outcome Measures
Name Time Method Palmitic acid in different plasma fractions 14-20 days Measured by liquid gas chromatography (GC)
- Secondary Outcome Measures
Name Time Method Even-chain saturated fatty acids (i.e. 14:0, 18:0) and monounsaturated fatty acids 14-20 days Measured by liquid GC
Trial Locations
- Locations (1)
Uppsala univeristy hospital
🇸🇪Uppsala, Sweden