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Effect of Green Tea (Epigallocatechin Gallate) on Albuminuria in Patients With Diabetic Nephropathy.

Phase 2
Completed
Conditions
Diabetic Nephropathy
Hypertension
Interventions
Registration Number
NCT01923597
Lead Sponsor
University of Campinas, Brazil
Brief Summary

The purpose of this study is to determine the safety and effect of green tea (epigallocatechin gallate) in albuminuria in diabetic patients and nephropathy.

Detailed Description

Clinical, prospective, randomized, double-blind, placebo-controlled, with analysis by intention to treat.

50 individuals will be selected with a diagnosis of diabetes / hypertension and has been followed in Diabetic Nephropathy Clinic of the Faculty of Medical Sciences, University of Campinas (UNICAMP). Participants are divided into 02 groups: 1) 25 patients treated with maximum dose of ACE-I and / or angiotensin II receptor blocker (ARBs) + Placebo (absence of epigallocatechin gallate) and 2) treated 25 patients with maximum dose of ACE-I and / or ARBs + green tea (epigallocatechin gallate).

The patients will receive four capsules Polyphenol E - epigallocatechin gallate (Polyphenon Pharma, NY) per day, corresponding to 800 mg of epigallocatechin gallate (EGCG), or placebo (no epigallocatechin gallate) for 3 months. Patients will not be aware of the treatment they are receiving. The subjects will be allocated for the treatment or placebo, stratified by sex. To avoid the influence of researchers, the randomization list will be generated and maintained by trained personnel in a different location from the study. Before treatment and immediately after 3 months of treatment will be obtained in the primary outcome measures (albuminuria) and secondary (plasma metabolites of flavonoids, level of urinary F2-isoprostane and 8-hydroxydeoxyguanosine). In these same times will be obtained: blood biochemistry (glucose, glycosylated hemoglobin, urea, creatinine, sodium, potassium, blood count, calcium, phosphorus, cholesterol, LDL, HDL, triglycerides, uric acid), 3 samples of first morning urine to determine albuminuria, glomerular filtration rate (GFR), blood pressure measurement of 24 h, physical examination, weight, blood pressure and heart rate. Adherence to the study will be evaluated by weekly phone and the expected increase in plasma of flavonoids using the green tea (epigallocatechin gallate). The antihypertensive drug may be adjusted to obtain the desired pressure (\<130/80 mmHg).

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
50
Inclusion Criteria
  • 18 years or more
  • prior diagnosis of diabetes mellitus (DM)
  • persistent micro-or macroalbuminuria (urinary albumin excretion> 30 mg / g creatinine (AUC) in 3 consecutive measurements on different days)
  • glycated hemoglobin <10%
  • maximum dose of ACE-I and / or ARBs.
Exclusion Criteria
  • diagnosis of autoimmune diseases, HIV, hepatitis, cancer, inflammatory disease
  • pregnant or lactating patients
  • glomerular filtration rate (GFR) <30 ml/min/1, 73m2 (estimated by the MDRD and the Cockcroft-Gault formula)
  • presence of kidney disease unrelated to diabetes
  • chronic urinary tract infection
  • diagnosis of congestive heart failure (CHF) New York Heart Association (NYHA) class III or IV
  • recent history (<6 months) unstable angina, myocardial infarction, stroke, coronary intervention
  • history of alcohol and / or drugs
  • mental incapacity to understand the informed consent
  • intolerance to green tea

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Green tea extractGreen tea extractPatients will receive four capsules ( one capsula = 200mg of epigallocatechin gallate) of green tea extract per day for 3 months.
Placebo (celulose)Green tea extractPatients will receive four capsules of placebo (celulose) daily for 3 months.
Primary Outcome Measures
NameTimeMethod
Effect of green tea (epigallocatechin gallate) in albuminuria in diabetic patients and nephropathy.After 3 months of treatment

Mean of 3 urinary albumin to creatinine ratio.

Secondary Outcome Measures
NameTimeMethod
Effect of green tea (epigallocatechin gallate) on oxidative stress in patients with diabetic nephropathy.After 3 months of treatment

Urinary levels of F2-isoprostane and 8-hydroxydeoxyguanosine

Effect of green tea (epigallocatechin gallate) on plasma metabolites of flavonoids in patients with diabetic nephropathy.After 3 months of treatment

Plasma levels of epigallocatechin gallate and epicatechin.

Effect of green tea (epigallocatechin gallate) on blood pressure in patients with diabetic nephropathy.After 3 months of treatment

24 h blood pressure monitoring

Effect of green tea (epigallocatechin gallate) on plasma lipids in patients with diabetic nephropathy.After 3 months of treatment

Plasma levels of triglycerides.

Effect of green tea (epigallocatechin gallate) on blood glucose control in patients with diabetic nephropathy.After 3 months of tretatment

Glycemia and glycated hemoglobin

Trial Locations

Locations (1)

Unicamp

🇧🇷

Campinas, São Paulo, Brazil

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