The Effects of Essential and Non-Essential Amino Acids on Post-Exercise Muscle Collagen Synthesis in Young Men: a Randomized Controlled Trial
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 17
- 试验地点
- 1
- 主要终点
- Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis
研究概览
简要总结
The purpose of this study is to assess which amino acids are affecting acute muscle collagen synthesis in response to supplementation and exercise.
详细描述
This is a double-blinded, randomized controlled, parallel design study, with 3 treatment groups (placebo, collagen peptide, and essential amino acid). The study will involve 3 visits: a baseline testing/familiarization session, and 2 exercise trials which will include muscle biopsies, blood draws, and the infusion of a tracer isotope in order to measure muscle protein synthesis.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Triple (Participant, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Can fluently read and write in English
- •18 - 35 years
排除标准
- •Diabetes, cardiovascular disease, or current pharmaceutical therapy
- •Medical conditions impacting ability to undertake strenuous physical activity
- •Lower body resistance training in the previous 6 months
- •BMI > 30
- •Currently smoking with cigarettes
- •Allergy to any study supplements
- •Currently taking any protein, amino acid, or collagen supplement
- •Participate in greater than 2 hours of weekly vigorous exercise
研究组 & 干预措施
Essential Amino Acid
Participants will consume 1 dose of: 15 g crystalline essential amino acid supplement (Pure Encapsulation Essential Aminos 180) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water
干预措施: Essential Amino Acid (Dietary Supplement)
Maltodextrin Placebo
Participants will consume 1 dose of:15 g maltodextrin (Canadian Protein Maltodextrin) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water
干预措施: Maltodextrin Placebo (Dietary Supplement)
Collagen Peptide
Participants will consume 1 dose of: 15g collagen peptide (Gelita TENDOFORTE) + 80 ml (48 mg) Vitamin C (Ribena Blackcurrant) + 250 ml water
干预措施: Collagen Peptide (Dietary Supplement)
结局指标
主要结局
Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis
时间窗: 2 - 4 hours post-exercise
Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.
Fractional synthetic rate (change over time) Muscle Collagen Synthesis
时间窗: 2 - 4 hours post-exercise
Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.
Pro-Collagen Type I N-Propeptide (PINP)
时间窗: 4 hours post exercise
Osteoblastic marker of bone collagen synthesis
Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis
时间窗: 0 - 4 hours post-exercise
Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.
Fractional synthetic rate (change over time) of Myofibrillar Protein Synthesis
时间窗: 0 - 2 hours post-exercise
Fraction of the myofibrillar enriched protein from muscle biopsy per unit time.
Fractional synthetic rate (change over time) Muscle Collagen Synthesis
时间窗: 0 - 4 hours post-exercise
Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.
Fractional synthetic rate (change over time) Muscle Collagen Synthesis
时间窗: 0 - 2 hours post-exercise
Fraction of the intramuscular collagen enriched protein from muscle biopsy per unit time.
Pro-Collagen Type I N-Propeptide (PINP)
时间窗: 1 hour prior to exercise
Osteoblastic marker of bone collagen synthesis
次要结局
- PCR Gene Expression of Muscle Collagen Genes (Types I, III, IV)(0 - 4 hours post-exercise)
- Muscle mechanistic target of rapamycin Pathway Activation(0 - 4 hours post-exercise)
- Pro-Collagen Type III N-Propeptide (PIIINP)(4 hours post exercise)
- Plasma Amino Acids Response to Supplementation(0 - 4 hours post-exercise)
- Pro-Collagen Type III N-Propeptide (PIIINP)(1 hour prior to exercise)
研究者
Cameron Mitchell
Assistant Professor
University of British Columbia