Gene Expression in Nutritional Intervened Athletes
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 26
- 试验地点
- 2
- 主要终点
- Gene expression analysis
研究概览
简要总结
Background: Exercise represents an important challenge for the homeostasis of the entire body that occurs on a cellular and systemic level in which micronutrients play an important role in regulating the processes that sustain athletic performance. Objective: The investigators measured changes in gene expression of whole blood in a group of athletes and sedentary participants and compared gene modulation before and after nutritional intervention with micronutrients. Methods: Blood samples were taken from thirteen athletes and thirteen sedentary age- and gender-matched participants. The study design was carried out over a period of 4 months where three time points were established: (T0) baseline conditions in the sedentary and athlete groups; (T2) after two months of supplementation; (T4) after two months in the absence of nutritional supplementation. Differential gene expression was evaluated in 112 genes using RT-qPCR analysis with the QuantStudioTM 12K Flex Real-Time PCR System.
详细描述
Background: Exercise represents an important challenge for the homeostasis of the entire body that occurs on a cellular and systemic level in which micronutrients play an important role in regulating the processes that sustain athletic performance. Objective: The investigators measured changes in gene expression of whole blood in elite athletes and sedentary subjects and compared gene modulation before and after nutritional intervention with micronutrients. Methods: Thirteen elite athletes and thirteen sedentary controls were enrolled in the present study. Blood samples were taken from athletes and sedentary age- and gender-matched subjects. The study design was carried out over a period of 4 months where three time points were established: (T0) baseline conditions in the sedentary and handball groups; (T2) after two months of supplementation; (T4) after two months in the absence of nutritional supplementation. Differential gene expression was evaluated in 112 genes using RT-qPCR analysis with the QuantStudioTM 12K Flex Real-Time PCR System.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 20 Years 至 25 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •To pass a recruitment medical examination consisting of a clinical examination and recording the participant's medical history.
- •Non-smoker and not being on a medication regimen.
- •Written informed consent prior to participation.
- •With regard to the sedentary controls, none of the subjects reported at least 150 min·wk-1 of moderate-intensity or 75 min·wk-1 of vigorous-intensity aerobic physical activity necessary for substantial health benefits.
- •With regard to athletes, to be involved in a training regime including both indoor exercise and integrated conditioning exercises in addition to competing in matches on weekends.
排除标准
- 未提供
研究组 & 干预措施
Control Group
Sedentary: Multicentrum® 2 months. Subjects maintaining the multivitamin supplement (Multicentrum® (Pfizer, Spain) 1 tablet/day) during 2 months
干预措施: Multicentrum (Dietary Supplement)
Experimental Group
Athletes: Multicentrum® 2 months. Subjects maintaining the multivitamin supplement (Multicentrum® (Pfizer, Spain) 1 tablet/day) during 2 months
干预措施: Multicentrum (Dietary Supplement)
结局指标
主要结局
Gene expression analysis
时间窗: 6 months
112 genes using RT-qPCR analysis with the QuantStudioTM 12K Flex Real-Time PCR System. Relative quantification was determined using the 2-ΔCT method. Cut-off punctuation was established as 1.5.
次要结局
- Biochemical parameters - Urea(At baseline)
- Anthropometric assessment - Muscle mass(6 months)
- Biochemical parameters - Glucose(At baseline)
- Biochemical parameters - Uric acid(At baseline)
- Biochemical parameters - Triglycerides(At baseline)
- Biochemical parameters - Total cholesterol(At baseline)
- Biochemical parameters - Total proteins(At baseline)
- Anthropometric assessment - Fat percentage(6 months)
- Anthropometric assessment - Fat free mass(6 months)
- Anthropometric assessment - Height(6 months)
- Anthropometric assessment - Weight(6 months)
- Anthropometric assessment - BMI(6 months)
- Biochemical parameters - Creatinine(At baseline)
- Biochemical parameters - Transferrin(At baseline)
- Biochemical parameters - Albumin(At baseline)
研究者
Elena Planells del Pozo
University Professor
Universidad de Granada