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A Feasibility, Dose-Escalation Study Using Intracerebral Microdialysis to Assess the Neuropharmacodynamics of Temsirolimus in Patients With Primary or Metastatic Brain Tumors

Phase 1
Completed
Conditions
Brain and Central Nervous System Tumors
Metastatic Cancer
Interventions
Other: cytokine levels
Other: pharmacological study
Registration Number
NCT00784914
Lead Sponsor
City of Hope Medical Center
Brief Summary

RATIONALE: Temsirolimus may stop the growth of tumor cells by blocking some of the enzymes needed for cell growth. Studying samples of blood and tumor tissue from patients with cancer in the laboratory may help doctors learn more about how this treatment is used by the body.

PURPOSE: The purpose of this study is to evaluate the feasibility of using a microdialysis catheter to see what effect temsirolimus has on various biological substances associated with brain tumors over time.

Detailed Description

OBJECTIVES:

Primary

* Determine the feasibility of using a microdialysis catheter with a high cut-off membrane to perform neuropharmacodynamics (nPD) assessment of targeted therapy with a mammalian target of rapamycin (mTOR) inhibitor, where nPD is defined as changes in intracerebral levels of vascular endothelial growth factor (VEGF), interleukin-1ß (IL-1ß), and other cytokines.

Secondary

* Assess the relationship between temsirolimus dose and changes in intracerebral levels of VEGF, IL-1ß, and other cytokines.

* Compare changes in intracerebral cytokine levels to changes in systemic cytokine levels.

* Assess the relationship between the degree of microvascular proliferation and the tensin homologue deleted on chromosome 10 (PTEN) status in tumor tissue.

* Assess the relationship between changes in intracerebral cytokine levels after treatment with temsirolimus.

OUTLINE: Two cohorts of 6 patients will be enrolled in this study. All patients undergo debulking craniotomy or stereotactic biopsy and a placement of a intracerebral CMA 71 microdialysis (ICMD) catheter. Patients then are assigned to 1 of 2 treatment cohorts.

* Cohort 1: Patients do not receive temsirolimus. Dialysate samples will be collected at regular intervals during the 96 hours following placement of the catheter as well as serial blood samples to measure levels of cytokines, chemokines and growth factors that occur after neurosurgery.

* Cohort 2: Beginning 48 hours after surgery, patients receive a single 200 mg dose of temsirolimus IV. Dialysate samples will be collected at regular intervals during the 96 hours following placement of the catheter as well as serial blood samples to measure levels of cytokines, chemokines and growth factors that occur after neurosurgery. Plasma levels of temsirolimus and sirolimus will also be measured from the serial blood samples.

After completion of study therapy and removal of ICMD catheter, patients are followed for 30 days.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
12
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Cohort 1cytokine levelsPatients do not receive temsirolimus.
Cohort 2pharmacological study48 hours after surgery, patients receive one 200 mg dose of temsirolimus IV.
Cohort 2cytokine levels48 hours after surgery, patients receive one 200 mg dose of temsirolimus IV.
Cohort 2temsirolimus48 hours after surgery, patients receive one 200 mg dose of temsirolimus IV.
Primary Outcome Measures
NameTimeMethod
Feasibility of using a microdialysis catheter to assess the neuropharmacodynamics (nPD) of temsirolimusEvery 6 hours for 96 hours after confirmation that the microdialysis catheter is placed appropriately
Changes in intracerebral levels of vascular endothelial growth factor (VEGF), interleukin-1ß (IL-1ß), and other cytokinesEvery 6 hours for 96 hours after confirmation that the microdialysis catheter is placed appropriately
Secondary Outcome Measures
NameTimeMethod
Relationship between temsirolimus dose and changes in intracerebral levels of VEGF, IL-1ß, and other cytokinesEvery 6 hours for 96 hours after confirmation that the microdialysis catheter is placed appropriately
Relationship between the degree of microvascular proliferation and the tensin homologue deleted on chromosome 10 (PTEN) status in tumor tissue30 days after placement of the microdialysis catheter.
Relationship between changes in intracerebral cytokine levels after treatment with temsirolimusEvery 6 hours for 96 hours after confirmation that the microdialysis catheter is placed appropriately
Compare changes in intracerebral cytokine levels to changes in systemic cytokine levels.Every 6 hours for 96 hours after confirmation that the microdialysis catheter is placed appropriately

Trial Locations

Locations (1)

City of Hope Comprehensive Cancer Center

🇺🇸

Duarte, California, United States

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