Lipids and Energy Study: Optimizing the Analysis of Lipids and Energy Metabolism in Blood Samples

Recruiting

• Conditions: Healthy

• Registration Number: NCT04981288
• Lead Sponsor: Ohio State University

Brief Summary

The proposed research is a feasibility pilot trial designed to optimize the remote collection dried blood spots (DBS) from a finger stick and the analysis of fatty acid from the DBS and to optimize the analysis of mitochondria activity (or energy metabolism) from peripheral blood mononuclear cells (PBMC) or white blood cell samples. Participants in this study will provide a blood sample from venous blood draw and finger sticks. One finger stick collection will be completed remotely using a DBS kit that is sent through the mail while the other finger stick collection will be completed at the research site. These samples will be use to optimize the method for measuring fatty acids and mitochondria function as well as measure other lipids, insulin, glucose and markers of inflammation using established protocols. Demographic, dietary, sleep, and physical activity information will be measured using questionnaires, while height and weight will be measured to calculate body mass index (BMI).

Detailed Description

The primary objectives of the study are

* To optimize remote blood collection and laboratory analysis of polyunsaturated fatty acids (PUFAs) in dried blood spot (DBS) samples

* To optimize the analysis of mitochondria function in fresh and frozen peripheral blood mononuclear cells (PBMC)

Study Timeline

• Study Start: 2021-07-02
• Study First Submitted: 2021-07-15
• Study First Submitted QC: 2021-07-26
• Study First Posted: 2021-07-28
• Status Verified: 2025-02
• Last Update Submitted: 2025-02-13
• Last Update Posted: 2025-02-17
• Primary Completion: 2025-08-01
• Study Completion: 2025-12-01

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

• 18 years of age or older
• Nonsmoking

Exclusion Criteria

• Diabetes

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Optimize mitochondria function measurements in fresh and frozen blood	1 Day	Measuring oxygen consumption rate in peripheral blood mononuclear cells using seahorse analyzer
Optimize remote blood collection and laboratory analysis of in dried blood spot samples	2 Days	Collecting dried blot spots remotely and in the research setting and venous blood draw in the research setting to compare fatty acid composition

Secondary Outcome Measures

Name	Time	Method
Assess the relationship of mitochondria function in and cardiolipin in peripheral blood mononuclear cells	1 Day	Determine mitochondria function in peripheral blood mononuclear cells by measuring oxygen consumption rate and determining its relationship to the mitochondrial phospholipid cardiolipin
Assess the relationship of mitochondria function and markers of inflammation	1 Day	Determine mitochondria function in peripheral blood mononuclear cells by measuring oxygen consumption rate and determining its relationship to markers of inflammation in plasma
Assess the relationship of mitochondria function in peripheral blood mononuclear cells and markers of glycemia	1 Day	Determine mitochondria function in peripheral blood mononuclear cells by measuring oxygen consumption rate and determining its relationship to markers of glycemia in serum
Assess the relationship of mitochondria function and fatty acid metabolites	1 Day	Determine mitochondria function in peripheral blood mononuclear cells by measuring oxygen consumption rate and determining its relationship to the fatty acid metabolites in plasma

Trial Locations

Locations (1)

The Ohio State University Human Nutrition Laboratory; 🇺🇸 Columbus, Ohio, United States