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Study Assessing RLT Using [177Lu]Lu-PentixaTher for Relapsed/Refractory CXCR4+ Acute Leukemia.

Phase 1
Recruiting
Conditions
Acute Leukemia
Interventions
Drug: Experimental drug [177Lu]Lu-PentixaTher
Registration Number
NCT06356922
Lead Sponsor
Nantes University Hospital
Brief Summary

CXCR4 inhibition may represent a new therapeutic strategy in acute leukemia (AL) patients, not only by increasing chemosensitivity but also by preventing relapse of the disease by disruption of the interaction of residual leukemic cells with the bone marrow niche. Radiolabeled CXCR4 ligands have been developed for PET imaging (68Ga-PentixaFor; INN: Gallium (68Ga) boclatixafortide) and radioligand therapy (RLT) (\[177Lu\]Lu-PentixaTher/\[90Y\]Y-PentixaTher). \[177Lu\]Lu and \[90Y\]Y-PentixaTher have been tested in three multiple myeloma patients in named-patient use with a remarkable efficacy in 2 patients (Herrmann, 2016). Moreover, feasibility of CXCR4 PET imaging in AML was reported, providing a framework for future theranostic approaches targeting the CXCR4/CXCL12-defined leukemia-initiating cell niche (Herhaus, 2016).

Here a Phase I/II study to determine maximal tolerated dose (MTD) of a RLT using \[177Lu\]Lu-PentixaTher in relapsed/refractory AL was designed. This will be a standard phase I/II 3+3 dose escalation study. Five dose levels will be tested, so 6 to 21 patients have to be included in the study.

Detailed Description

Not available

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
21
Inclusion Criteria
  • Age ≥ 18 years
  • AML/ALL (OMS) with >5% of blasts in bone marrow (with or without extramedullary localisation)
  • CXCR4+ (ratio >2/isotypic control) at the time of pre-inclusion
  • All previously treated AML/ALL patients who have experienced relapse or treatment failure with no alternative treatment
  • At least 15 days since previous treatment
  • Eastern Cooperative Oncology Group (ECOG) performance status < 2 (Annex 6)
  • eGFR ≥ 50 ml/min by MDRD or CKDEPI
  • ASAT or ALAT > 5 upper normal value (except in case of documented presence of leukemia in the liver)
  • Serum bilirubin ≤ 30 µmol/l
  • Negative pregnancy test documented prior to enrolment (for females of childbearing potential)
  • Agree to use an effective form of contraception with sexual partners throughout study participation (for female and male patients who are fertile)
  • No active cardiac dysfunction (LVEF > 45%)
  • DLCO >40%
  • Written informed consent
  • Be willing and able to comply with scheduled visits and study procedures
  • Affiliation with French social security system or beneficiary from such system
Exclusion Criteria
  • Meningeal involvement
  • HIV positive
  • Active Hepatitis B or C
  • Active infection within 7 days of starting treatment
  • Previous or concurrent second malignancy except for adequately treated basal cell carcinoma of the skin, curatively treated in situ carcinoma of the cervix, curatively treated solid cancer, with no evidence of disease for at least 1 year
  • Any psychological, familial, sociological or geographical condition potentially hampering compliance with the study protocol and follow-up schedule
  • Participation at the same time in another study in which investigational drugs are used
  • Patient with contra-indications to Rhu-EPO, Rhu-GCSF, allopurinol, rasburicase, anti-histamines and corticosteroids
  • Absence of written informed consent
  • Pregnant or child breast feeding woman
  • Patient under guardianship or trusteeship
  • Patient under judicial protection

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
[177Lu]Lu-PentixaTherExperimental drug [177Lu]Lu-PentixaTherInjection of \[177Lu\]Lu-PentixaTher
Primary Outcome Measures
NameTimeMethod
Safety of RLT using one injection of [177Lu]Lu-PentixaTherBetween Week 4 and Week 6

Number of participants with treatment-related adverse events as assessed by CTCAE v5.0

ToleranceBetween Week 4 and Week 6

Tolerance of the RLT will be evaluated by dosimetry studies, especially in terms of renal and hepatic doses delivered

Secondary Outcome Measures
NameTimeMethod
Overall response rateBetween Week 4 and Week 6

Response evaluation (CR, CRp and PR) after the infusion of \[177Lu\]Lu-PentixaTher

Complete response rateBetween Week 4 and Week 6

Response evaluation (CR, CRp) after the infusion of \[177Lu\]Lu-PentixaTher

Overall survivalMonth 12

Time interval from the date from initial of study treatment (D0) until the date of last follow-up or death

Leukemia-free survivalMonth 12

Time interval from the date of documented complete response (CR, CRp) until the date of last follow-up, death or relapse

Minimal residual diseaseMonth 12

CXCR4 ratio by flow cytometry after \[177Lu\]Lu-PentixaTher

Whole-body biodistributionBetween Week 4 and Week 6

Serial whole body scintigraphies

Plasma uptakeBetween Week 4 and Week 6

The activity in each plasma sample will be determined by counting 0,2 ml of plasma in a calibrated gamma counter with an appropriate window setting. The maximal uptake (%) and area under the curve (AUC) of \[ 177Lu\]Lu-PentixaTher at the target lesion, organs and blood will be determined

Radiation dosimetryBetween Week 4 and Week 6

Whole body quantitative scintigraphies

Renal safetyMonth 12

Renal safety will be assessed by measuring urinalysis

Correlation between different cytokines and toxicityMonth 12

FLT3 and IL6 serum level

Factors associated responseMonth 12

Responses will be evaluated 4/6 weeks after the infusion of \[177Lu\]Lu-PentixaTher (Day 0): Complete remission (CR) is defined by normalization of the blood and the bone marrow with \< or = 5% of blasts, neutrophil count \> 1.109 /l and platelet count \>100 Giga/l. CR with incomplete platelets recovery (CRp) is defined as for CR including platelet transfusion independence but with platelet count remaining below 100 Giga/l. Partial response (PR) is defined by blast clearance ≥50% in blood or bone marrow or bone marrow with \> 5% and \< 20 % of blasts

Exploratory outcome measure = Identification of biological biomarkersMonth 12

Different cytokines including FLT3 and IL6 serum levels will be monitored by serial blood sampling at D1, D8, D15, D22 as well as during the monitoring visits at 1 month, and only FLT3 and IL6 at 3, 6, 9 and 12 months

Trial Locations

Locations (4)

CHU de Bordeaux

🇫🇷

Bordeaux, Gironde, France

CHU de Nantes

🇫🇷

Nantes, Loire-Atlantique, France

CHU d'Angers

🇫🇷

Angers, Maine et Loire, France

CHU de Clermont-Ferrand

🇫🇷

Clermont-Ferrand, Puy de Dôme, France

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