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Modulation Contrast Microscopy Imaging for Oocyte Quality Assessment

Conditions
Oocyte Competence
Registration Number
NCT03683290
Lead Sponsor
Center for Reproductive an Genetic Health
Brief Summary

The present study aims to describe novel non-invasive parameters related to the competence of female reproductive cells (oocytes). In a fertility unit, human reproductive cells and embryos are subjected to different imaging technologies. Images can easily be obtained without disturbing the microenvironment during different standard procedures. Turning images into quantifiable data might provide a further understanding on oocyte behavior and performance in the laboratory.

Detailed Description

Not available

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
200
Inclusion Criteria
  • Intracytoplasmic sperm injection
  • Embryo culture with subsequent fresh embryo transfer
Exclusion Criteria
  • Conventional IVF

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Implantation6 weeks gestation

Number of gestational sacs seen on ultrasound scan

Secondary Outcome Measures
NameTimeMethod
Fertilisation17 ± 1 hours post insemination

Appearance of two pronucleii after insemination

Euploidy2-3 weeks post insemination

Number of chromosomes (if available, after PGT-A)

Nuclear maturity36-41 post hCG

Presence of meiotic spindle (if available, PolScope)

Embryo quality clevage68 ± 1 hours post insemination

Quality after three days of culture

Live Birth40 weeks gestation

Baby to be born after embryo transfer

Embryo quality Blastocyst116 ±2 hours post insemination

Quality at the blastocyst stage

Trial Locations

Locations (1)

Centre for Reproductive and Genetics Health

🇬🇧

London, United Kingdom

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