Acute Resistance Exercise and Hydrolyzed Collagen Supplementation
- Conditions
- DietFoodNutrition
- Interventions
- Dietary Supplement: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exerciseDietary Supplement: Consumption of three different doses of hydrolyzed collagen (HC) with resistance exercise
- Registration Number
- NCT06236659
- Lead Sponsor
- Rob Erskine
- Brief Summary
This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in middle-aged males and females.
- Detailed Description
This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy middle-aged males and females.
Healthy middle-aged males and females ingested 0 grams, 15 grams or 30 grams HC with 50 milligrams vitamin C 1h prior to performing four sets' leg press RE at 10-repetition maximum load, after which they rested for six hours. Blood samples were collected throughout each trial to analyse procollagen type Ⅰ N-terminal propeptide (PⅠNP, a biomarker of collagen synthesis) and β-isomerized C-terminal telopeptide of type I collagen (β-CTX, a biomarker of collagen breakdown) concentration, and the concentration of 18 amino acids that constitute collagen.
This is the first study to investigate the combined effect of different doses HC with high-intensity RE on whole body collagen synthesis in middle-aged men and women. If 30 grams HC intake with RE does augment collagen synthesis more than RE alone, this suggests that long-term HC intake with chronic RE would be beneficial for tendon health, because collagen is the most abundant protein in tendon.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 10
- At least 12 months experienced in resistance exercise (including weekly lower body exercise)
- Free from musculoskeletal injury
- Having a history of patellar tendon pathology
- Vegan
- Consumed nutritional supplements or medication purported to have beneficial effects on muscle-tendon properties (e.g. antioxidants, protein, etc.)
- Had a sustained a lower limb injury in the previous six months
- Smoker/vaper
- Having reached menopause (only for middle-aged female participants)
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Intervention for middle-aged women: Consumption of hydrolyzed collagen (HC) with resistance exercise Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise The intervention procedure is exactly same as Arm 1 except for the number of visits and doses of HC. Middle-aged female participants were asked to visit the laboratory on the day of highest oestrogen (i.e. ovulation) and provided with 0 g or 30 g HC. Dates for the trials were determined based on self-report of onset of menses and previous menstrual cycle length. Intervention for middle-aged men: Consumption of hydrolyzed collagen (HC) with resistance exercise Consumption of three different doses of hydrolyzed collagen (HC) with resistance exercise Middle-aged male participants consumed one of three different HC doses (0 grams, 15 grams, or 30 grams) with 4 sets of 10 repetitions of leg press exercise at 10-repetition maximum load in a random order and a seven-day wash-out period interspersed between each trial.
- Primary Outcome Measures
Name Time Method Change in a marker of collagen synthesis At rest immediately prior to HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE Serum procollagen type Ⅰ N-terminal propeptide (PⅠNP) concentrations were measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistance exercise (RE) during all interventions.
Change in a marker of collagen breakdown At rest, immediately prior to HC ingestion, 0.5-hour post RE, 2-hour post RE and 6-hour post RE Plasma β-isomerized C-terminal telopeptide of type I collagen (β-CTX) concentrations were measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistance exercise (RE) during all interventions.
- Secondary Outcome Measures
Name Time Method Estrogen concentrations in women in each trial At rest immediately prior to HC ingestion Concentrations of estrogen (17β-estradiol) was measured in each intervention.
Changes in amino acids concentrations in blood At rest immediately prior to HC ingestion, 0.5-hour post HC ingestion, 1-hour post HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE Concentration of serum amino acids that constitute collagen was measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistance exercise (RE) during all interventions.
Trial Locations
- Locations (1)
Dr Rob Erskine
🇬🇧Liverpool, United Kingdom