Expression of DMBT1 in Colorectal Cancer Patients

Completed

• Conditions: Colorectal Cancer; DMBT1 Protein, Human
• Interventions: Other: Detection of the expression of DMTB1 by immunohistochemistry

• Registration Number: NCT02901600
• Lead Sponsor: Lille Catholic University

Brief Summary

Although colorectal cancer is a preventable and curable disease if early stage tumors are removed, it is still the fourth cause of cancer worldwide and the second leading cause of death in many industrialized countries. The 5-year survival is about 55% often due to a late detection. Then, the identification of sensitive and specific molecular markers is therefore a major challenge for early diagnosis and prognosis of this disease.

Preliminary work have reported variations in the expression of DMBT1 (deleted in malignant brain tumor 1), a glycoprotein co-secreted with mucins in the light of the glands, during several stages of colon carcinogenesis. The goal of this study is to study by mass spectrometry (MS), alterations in the repertoire of glycosylation of mucins from colorectal tumors of various stages, grades, and recurrence status.

Detailed Description

Not available

Study Timeline

• Study Start: 2013-06
• Primary Completion: 2015-06
• Study Completion: 2015-11
• Status Verified: 2016-09
• Study First Submitted: 2016-09-12
• Study First Submitted QC: 2016-09-12
• Last Update Submitted: 2016-09-12
• Study First Posted: 2016-09-15
• Last Update Posted: 2016-09-15

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 145

Inclusion Criteria

• for colon cancer: patients surgically treated for colorectal cancer at the Group of hospitals of the Catholic Institute of Lille.
• for adenomatous polyps : patients treated for adenomatous polyps at the Group of hospitals of the Catholic Institute of Lille.
• for controls: patients surgically treated for diverticular disease at the Group of hospitals of the Catholic Institute of Lille.

Exclusion Criteria

• none

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Patients with colorectal cancer	Detection of the expression of DMTB1 by immunohistochemistry	Fresh tissue samples from individuals with colorectal carcinoma taken from the tumor as well as from resection margins will be used for the detection of a potential marker of carcinogenesis.
Patients with adenomatous polyps	Detection of the expression of DMTB1 by immunohistochemistry	Fresh tissue samples from individuals with adenomatous polyps will be used for the detection of a potential marker of carcinogenesis.
Control patients	Detection of the expression of DMTB1 by immunohistochemistry	Fresh tissue samples from individuals without colorectal carcinoma will be used as control.

Primary Outcome Measures

Name	Time	Method
Semi-quantitative determination of the histological expression of DMBT1	at baseline	The expression of DMTB1 will be determined by observation of the slides after immunohistochemistry using an anti-DMBT1 antibody
Determination of the histological localisation of DMBT1	at baseline	The localisation of DMTB1 will be determined by observation of slides after immunohistochemistry using an anti-DMBT1 antibody