Large-scale ex Vivo Expansion and Characterization of Synovial Membrane Mesenchymal Stem Cells (SM-MSC) and Infrapatellar Fat Pad Mesenchymal Stem Cells (IF-MSC) for Clinical Application
试验速览
- 阶段
- 不适用
- 发起方
- 入组人数
- 6
- 试验地点
- 1
- 主要终点
- Cell viability ratio of synovial membrane MSC and infrapatellar fat pad MSC from the first to the tenth cell passage
研究概览
简要总结
According to many recent research, mesenchymal stem cells (MSC) isolated from synovial membrane and infrapatellar fat pad have higher ex vivo proliferation capacity, multipotency and ability to undergo chondrogenesis.
Based on this concept, the investigators plan to get two removed tissues (synovial membrane and infrapatellar fat pad) from patients who have an operation of total knee replacement. The investigators are going to isolate and expand MSC from these two tissues respectively, and then compare the chondrogenesis ability for clinical application. In the other hand, the investigators plan to build techniques and procedures which comply with "Good Tissue Practices (GTP)"
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Prospective
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Patient who have an total knee replacement surgery
排除标准
- •Patient who don't sign the agreement
结局指标
主要结局
Cell viability ratio of synovial membrane MSC and infrapatellar fat pad MSC from the first to the tenth cell passage
时间窗: 6 months
Percentage expression of cluster differentiation 90,105, 34, 45 surface markers of synovial membrane MSC and infrapatellar fat pad MSC from the first to the tenth cell passage
时间窗: 6 months
Doubling time of synovial membrane MSC and infrapatellar fat pad MSC from the first to the tenth cell passage
时间窗: 6 months
次要结局
- Alcian blue staining of synovial membrane MSC and infrapatellar fat pad MSC(6 months)
- Tyep II collagen and aggrecan gene expression of synovial membrane MSC and infrapatellar fat pad MSC(6 months)
研究者
Chin-Hung Chang
MD. PhD.
Far Eastern Memorial Hospital