Microbial Biomarkers of EArly Pseudomonas Aeruginosa Colonization in CHildren With Cystic Fibrosis
Overview
- Phase
- Not Applicable
- Intervention
- collection of expectoration, stools and blood
- Conditions
- Cystic Fibrosis
- Sponsor
- University Hospital, Brest
- Enrollment
- 70
- Locations
- 11
- Primary Endpoint
- Presence of P. aeruginosa in bacterial sputum cultures collected at 36 months of age
- Status
- Active, not recruiting
- Last Updated
- 2 months ago
Overview
Brief Summary
The objective of this study is to evaluate the predictive nature of the biomarker Porphyromonas catoniae measured at the age of 12 months in the occurrence of colonization with Pseudomonas aeruginosa at 36 months of age in children with cystic fibrosis.
Detailed Description
This is a multicentric study in 3 phases: * Pre-inclusion: at the first visit to the CRCM (support for a positive screening confirmed by sweat test and genotyping CFTR) * Inclusion: possible between the 2nd visit to the CRCM (about 2 months old) and the 6th month * Follow-up: up to 36 months old. The pace of visits will be based on the usual follow-up rate of CF infants The clinical data as well as samples (expectorations, stools) will be collected on a monthly basis up to 6 months old and then every 2 months until one year old and finally quarterly until 3 years old. * Tracheo-bronchial secretions will be collected at the CRCM * Stools samples will be carried out by the parents prior to consultation with the CRCM * A blood collection will be carried out annually in an annual report.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Infants aged 6 months maximum at inclusion with a confirmed diagnosis of cystic fibrosis in its classical form (positive sweat test and/or two mutations of the CFTR gene from class I to III) -Children free from any antecedent of colonization to P. aeruginosa at the time of inclusion (certified by the microbiological history supplemented by a molecular test by qPCR according to the diagram of Le gal et al., 2013)---
- •Affiliation to the social security system
- •Consent signed by the holders of parental authority or the sole parent holding parental authority / and "oral" agreement of the second holder
Exclusion Criteria
- •Severe acute illness (other than cystic fibrosis) ongoing, or requiring surgery
- •Children unable to undergo the tests required by the protocol
- •Children whose parent(s) is/are minors
- •Children whose legal guardians do not have sufficient command of the French language
- •Children under exclusive parenteral nutrition
- •Refusal to participate in the study
Arms & Interventions
collection of expectoration, stools and blood
Intervention: collection of expectoration, stools and blood
Outcomes
Primary Outcomes
Presence of P. aeruginosa in bacterial sputum cultures collected at 36 months of age
Time Frame: 36 months
positive or negative
Secondary Outcomes
- Absolute amount of P. aeruginosa at different sampling times.(36 months)
- Level of dysbiosis(36 months)
- Absolute amount of P. catoniae in respiratory secretions at different sampling times and Delta between 12, 24 and 36 months.(36 months)
- Absolute amount of P. catoniae in stool at different sampling times and Delta between 12, 24 and 36 months.(36 months)
- Number, diversity, and quality of antibiotic resistance genes(36 Months)
- Levels of blood metabolites(36 Months)
- Pulmonary concentration of inflammatory markers(36 months)
- Constitutional SNPs(36 Months)
- Breastfeeding at each visit(36 Months)
- Dietary diversification at each visit(36 Months)
- Treatments received at each visit(36 Months)