Effects of Acute HIIT and MICT on Catestatin Dynamics in Sedentary Young Males: Selective Association With Post-Exercise Autonomic Recovery
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 20
- 试验地点
- 1
- 主要终点
- Serum Catestatin Concentration
研究概览
简要总结
Physical exercise acts as a major physiological stressor that alters cardiovascular and autonomic homeostasis. Catestatin (CTS), a bioactive peptide derived from chromogranin A (CgA), acts as a potent inhibitor of catecholamine secretion and modulates autonomic cardiovascular tone. However, the acute effects of distinct exercise modalities on circulating CTS dynamics and their associations with post-exercise autonomic recovery remain unclarified.
The purpose of this study is to investigate the acute responses of serum catestatin and chromogranin A to high-intensity interval training (HIIT) versus moderate-intensity continuous training (MICT) in healthy sedentary young males, and to evaluate their potential relationship with post-exercise heart rate recovery (HRR). Participants completed an initial incremental performance test followed by either a single HIIT session or a MICT session on a cycle ergometer. Serial blood samples and continuous cardiovascular measurements were obtained before, immediately after, and throughout a 60-minute post-exercise recovery period.
详细描述
Twenty healthy sedentary young adult males (aged 18-25 years) were assigned to either a high-intensity interval training group (HIIT, n=10) or a moderate-intensity continuous training group (MICT, n=10). Following an initial incremental maximal exercise test to determine individual exercise capacities, participants performed their assigned acute exercise protocol on a cycle ergometer separated by at least 48 hours.
The MICT session consisted of 30 minutes of continuous cycling at 60-70% of maximum heart rate (HRmax), whereas the HIIT protocol comprised four 4-minute intervals at 85-95% HRmax interleaved with 3-minute active recovery periods. Serial venous blood samples were collected at baseline (Pre), immediately post-exercise (0 min), and at 15, 30, 45, and 60 minutes post-exercise to analyze serum catestatin and chromogranin A concentrations via enzyme-linked immunosorbent assay (ELISA). Continuous heart rate and intermittent blood pressure were monitored throughout the exercise bouts and during the 60-minute recovery phase. Post-exercise autonomic regulation was assessed using 1-minute (HRR1) and 5-minute (HRR5) heart rate recovery indices.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 25 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Healthy male individuals aged 18-25 years
- •Body mass index (BMI) between 18.0 and 25.0 kg/m²
- •Sedentary lifestyle (no regular exercise engagement within the last 3 months; <90 min/week moderate-intensity or <60 min/week vigorous-intensity exercise)
- •Normal resting blood pressure (<140/90 mmHg)
- •Fasting plasma glucose between 60 and 100 mg/dL
- •Normal resting electrocardiogram (ECG) and pulmonary function tests (FEV1/FVC >80%)
- •Signed written informed consent
排除标准
- •Regular smoking or alcohol consumption
- •Any current or regular medication use
- •History or clinical signs of cardiovascular, respiratory, metabolic, or renal diseases
- •Symptoms suggestive of cardiopulmonary/metabolic disease (chest pain, shortness of breath, dizziness, syncope, ankle edema, palpitations, intermittent claudication)
- •Presence of known cardiovascular risk factors
- •Iron deficiency / anemia
- •Musculoskeletal limitations or contraindications to maximal cycle ergometer testing
研究组 & 干预措施
High-Intensity Interval Training (HIIT)
Participants completed an acute HIIT session on a cycle ergometer consisting of a 5-minute warm-up, followed by four 4-minute bouts at 85-95% of maximum heart rate (HRmax) interleaved with 3-minute active recovery periods, followed by a 5-minute cool-down.
干预措施: High-Intensity Interval Exercise (Other)
Moderate-Intensity Continuous Training (MICT)
Participants completed an acute MICT session on a cycle ergometer consisting of a 5-minute warm-up, followed by 30 minutes of continuous cycling at 60-70% of maximum heart rate (HRmax), followed by a 5-minute cool-down.
干预措施: Moderate-Intensity Continuous Exercise (Other)
结局指标
主要结局
Serum Catestatin Concentration
时间窗: Baseline (pre-exercise), immediately post-exercise (0 min), and at 15, 30, 45, and 60 minutes post-exercise.
Serum catestatin levels measured using enzyme-linked immunosorbent assay (ELISA) to evaluate acute exercise-induced dynamic responses.
次要结局
- Serum Chromogranin-A Concentration(Baseline (pre-exercise), immediately post-exercise (0 min), and at 15, 30, 45, and 60 minutes post-exercise.)
- Post-Exercise Heart Rate Recovery (HRR1 and HRR5)(At 1 minute and 5 minutes post-exercise.)
研究者
Orhan Sayın
MD
Firat University