Identifying Molecular Determinants for the Efficacy of Resistance Training for Improving Endurance Performance in Elite Cyclists
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 25
- 试验地点
- 2
- 主要终点
- Performance during a 15-minute all-out cycling test
研究概览
简要总结
The overall objective of the study is to identify molecular determinants of the efficacy of resistance training for improving endruance performance in elite cyclists
详细描述
For elite endurance athletes, the addition of resistance training to habitual endurance training routines generally leads to improved endurance performance. However, this is not always the case, with several individuals failing to respond in the expected manner. This inter-individual variation is likely associated with inherent cellular and molecular charateristics of skeletal muscle. Indeed, responses to resistance training seem to be associated with muscle biological characteristics such as muscle fiber composition and ribosomal content (during the early phase of resistance training).
The study will investigate the association between muscle fibre composition (type I vs type II muscle fiber proportions) / ribosomal content in m. vastus lateralis and the effects of 10 weeks of resistance training on endurance performance in 52 elite cyclists. Half the participants will perform resistance training in addition to their habitual endurance training routines, the other half will perform endurance training-only. Muscle biopsies will be sampled from m. vastus lateralis before and after the first two weeks of the intervention. For muscle fiber composition, the hypothesis is that larger proportions of type II fibers will be associated with more pronounced benefits of resistance training. For ribosomal content, the hypothesis is that higher rRNA content at baseline and larger increases in rRNA content during the initial part of the intervention will be associated with more pronounced benefits of resistance training. No changes are expected in the non-resistance training control group.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Double (Participant, Investigator)
盲法说明
Participants/investigators will be blinded to muscle biological charateristics until after clean-up of main outcome variables. Participants/investigators will be aware of intervention arm affiliation.
入排标准
- 年龄范围
- 18 Years 至 45 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •VO2max > 65 ml/kg/min (male participants)
- •VO2max > 50 ml/kg/min (female participants)
- •>7 hours of endurance training per week for the 6 months leading up to the study
排除标准
- •having conducted regular resistance training for the last 6 months leading up to the study
结局指标
主要结局
Performance during a 15-minute all-out cycling test
时间窗: Changes from before the intervention to immediately after the intervention
Mean power output measured during a 15-minute all-out cycling test
Performance during a 40-minute all-out cycling test
时间窗: Changes from before the intervention to immediately after the intervention
Mean power output measured during a 40-minute all-out cycling test
次要结局
- Total RNA in muscle tissue (changes after two weeks)(Changes from before the intervention to immediately after the initial two weeks of resistance training)
- Myonuclear number (changes after two weeks)(Changes from before the intervention to immediately after the initial two weeks of resistance training)
- Muscle fiber proportions (before intervention)(Before the intervention)
- Muscle fiber proportions (changes after two weeks)(Changes from before the intervention to immediately after the initial two weeks of resistance training)
- Muscle fiber cross-sectional area (before intervention)(Before the intervention)
- Myonuclear number (before intervention)(Before the intervention)
- rRNA expression (before intervention)(Before the intervention)
- Total RNA in muscle tissue (before intervention)(Before the intervention)
- Muscle fiber cross-sectional area (changes after two weeks)(Changes from before the intervention to immediately after the initial two weeks of resistance training)
- mRNA expression (before intervention)(Before the intervention)
- Sprint performance(Changes from before the intervention to immediately after the intervention)
- Fractional utilization of VO2max (incremental test)(Changes from before the intervention to immediately after the intervention)
- Fractional utilization of VO2max (15-minute performance test)(Changes from before the intervention to immediately after the intervention)
- Blood volume(Changes from before the intervention to immediately after the intervention)
- Hemoglobin mass(Changes from before the intervention to immediately after the intervention)
- Gross efficiency(Changes from before the intervention to immediately after the intervention)
- Hematocrit(Changes from before the intervention to immediately after the intervention)
- Maximal aerobic power output(Changes from before the intervention to immediately after the intervention)
- Maximal oxygen consumption(Changes from before the intervention to immediately after the intervention)
- Red blood cell volume(Changes from before the intervention to immediately after the intervention)
- rRNA expression (changes after two weeks)(Changes from before the intervention to immediately after the initial two weeks of resistance training)
- Maximal concentric force production(Changes from before the intervention to immediately after the intervention)
- mRNA expression (changes after two weeks)(Changes from before the intervention to immediately after the initial two weeks of resistance training)
- Power output at lactate threshold(Changes from before the intervention to immediately after the intervention)
- Plasma volume(Changes from before the intervention to immediately after the intervention)