Acute Effects of Instant Coffee in Capsule vs Beverage Form on Plasma Metabolites

Not Applicable

Completed

• Conditions: Coffee

• Registration Number: NCT07051291
• Lead Sponsor: National University of Singapore

Brief Summary

This study is designed to evaluate the acute effect of consuming a cup of instant coffee on circulating metabolites and to evaluate whether ingestion of instant coffee in the form of a capsule has a similar effect on circulating metabolites as compared to the conventionally prepared beverage.

Detailed Description

Not available

Study Timeline

• Study Start: 2020-09-22
• Primary Completion: 2020-10-10
• Study Completion: 2022-10-10
• Status Verified: 2025-06
• Study First Submitted: 2025-06-01
• Study First Submitted QC: 2025-06-30
• Last Update Submitted: 2025-06-30
• Study First Posted: 2025-07-04
• Last Update Posted: 2025-07-04

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 15

Inclusion Criteria

Healthy individuals aged 21-64 years old of Chinese ethnicity and are physically-abled, can participate in the research, and are able to understand English

Exclusion Criteria

• Pregnant or breastfeeding
• Certain medications including Zanaflex, Sirasalud, Ketanest S, Spravato and Ephedrine Sulfate
• Any pre-existing medical conditions, depression, anxiety, caffeine/lactose intolerant, fear of needles and/or blood disorders, allergy to coffee or bovine capsules
• Existing dietary restrictions which makes them unable to consume non-halal certified bovine gelatin (the capsule encasing the coffee powder)
• Recent history of major surgical operations in the past year
• Past or current user of oral/systemic hormone treatment or therapies
• Taken antibiotics in the last 3 months
• Taking nutritional supplements (that contains coffee/tea-related compounds naturally found in other plants)
• Not willing to travel to the University for the study
• Unable to commit to completing both study visits

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Primary Outcome Measures

Name	Time	Method
Caffeine and polyphenol metabolites	The period between the two study visits ranged between 7 to 28 days.	Each participant underwent two interventions, and blood was collected pre- and post- each intervention. Blood was allowed to clot at room temperature. After 30-40 min, serum was separated from entire blood by centrifugation (3000g, 15 min, 4°C) and aliquoted into tubes. All samples were processed on the same day of collection. A total of 7 biomarker compounds (trigonelline, paraxanthine, theophylline, theobromine, caffeine, cyclo(Leu-Pro), and cyclo(Pro-Val)) were quantified using a targeted method developed in-house. The quantification method was optimized by LC retention elution, parent-daughter quantifier mass transitions, and varying MS cone and collision energies (in V) to obtain clear peak separation and height. Multiple Reaction Monitoring (MRM) in positive electrospray ionization mode was performed on a Waters Xevo TQ-S Triple Quadrupole Mass Spectrometer system.

Trial Locations

Locations (1)

Saw Swee Hock School of Public Health, National University of Singapore; 🇸🇬 Singapore, Singapore