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Effect of Commute Traffic on Vascular Function

Not Applicable
Completed
Conditions
Cardiovascular Diseases
Interventions
Other: Filtered Air
Registration Number
NCT05454930
Lead Sponsor
University of Washington
Brief Summary

The investigators will assess the vascular effects of riding in a car in heavily trafficked roadways, and whether filtering the car's air reduces those effects.

Detailed Description

In this double-blind, crossover trial, randomized to order, recruited participants will be screened and then will accompany the study team on three typical commutes on separate days with sufficient washout (i.e., three weeks) between drives. One day this will occur with an effective filter system in the vehicle and the other two days without effective filtration; ineffective filtration is the most typical actual vehicle condition. Subjects will be unaware of the filtration conditions of the commute on that day. Each subject's experimental sessions will occur at the same time of day and will be separated by at least 21 days. The two sessions (either filtered air or traffic-derived entrained air) will be 120 minutes in duration and will encompass routes on heavily traveled roadways in the Seattle area. Before subjects begin the commute trials, they will go through the consent process and a series of screening tests. The study investigators will assess a series of measures regarding vascular function before and after each drive.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
16
Inclusion Criteria
  • healthy
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Exclusion Criteria
  • asthma,
  • high blood pressure,
  • diabetes,
  • high cholesterol,
  • other chronic conditions requiring ongoing medical care.
Read More

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Typical Commute, Filtered AirFiltered AirParticipants spend two hours in automobile, on heavily trafficked roadways, with active filtration of air pollutants
Primary Outcome Measures
NameTimeMethod
Change in Brachial Artery Diameter from Pre-Drive to Post-Drive30 minutes before 120 minute driving session; 30 minutes post driving session

Ultrasound assessment of brachial artery diameter, in millimeters.

Change in Blood Pressure from Pre-Drive to During-Drive and Post-drive10 minutes before driving session; 15, 30, 45, 60, 75, 90, 100, 120 minutes following start of (120 minute) driving session; 10 minutes, 1.5 hours, 3.5 hours, 6 hours, 24 hours after end of driving session.

systolic and diastolic blood pressure measured in mmHg

Change in Retinal Arteriolar Diameter from Pre-Drive to Post-Drive30 minutes before 120 minute driving session; 30 minutes post driving session

measured by retinal photography, image analysis, in micrometers

Gene Expression and DNA methylation30 minutes before 120 minute driving session; 30 minutes, 5 hour, and 24 hours post-driving session

analysis in circulating mononuclear cells; determined via fold change in abundance

Secondary Outcome Measures
NameTimeMethod
complete blood count with differential30 minutes before 120 minute driving session; 5 hour and 24 hours post-driving session

laboratory values for hematologic indices (cells/uL)

inflammatory marker interleukin-630 minutes before 120 minute driving session; 5 hour and 24 hours post-driving session

interleukin-6 determined by chemiluminescence (pg/mL)

inflammatory marker c-reactive protein30 minutes before 120 minute driving session; 5 hour and 24 hours post-driving session

C-reactive protein by immunoturbidimetry (ug/mL)

circulating stress hormone30 minutes before 120 minute driving session; 5 hour and 24 hours post-driving session

cortisol by chemiluminescence (ng/mL)

Trial Locations

Locations (1)

University of Washington Medical Center

🇺🇸

Seattle, Washington, United States

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