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Metabolism and Pharmacokinetics of Primaquine Enantiomers in Human Volunteers Receiving a Seven Day Dose Regimen

Phase 1
Completed
Conditions
Malaria
Glucose 6 Phosphate Dehydrogenase Deficiency
Interventions
Drug: Placebo
Drug: RPQ
Drug: SPQ
Registration Number
NCT03934450
Lead Sponsor
University of Mississippi, Oxford
Brief Summary

To investigate the comparative tolerability, metabolism and pharmacokinetics of individual enantiomers of PQ in healthy human volunteers, receiving study drug over the course of 7 days.

Detailed Description

The primary objective of this project is to investigate the comparative tolerability, metabolism and pharmacokinetics of individual enantiomers of PQ in healthy human volunteers. Based on the results of this study, if one enantiomer seems to show a better safety profile (in terms of hematological effects), an analogous study will be carried out in G6PD deficient individuals (under a separate protocol). The studies are primarily aimed at understanding the tolerability and safety of the enantiomers in G6PD deficiency. If one shows a better safety profile, ultimately the evaluation of its efficacy will be required.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
36
Inclusion Criteria
  • Normal, healthy adults aged 18 to 65 years
Exclusion Criteria
  • Known history of liver, kidney or hematological disease
  • Known history of cardiac disease, Non Sinus Rhythm arrhythmia or QT prolongation
  • Autoimmune disorders
  • Report of an active infection
  • Evidence of G6PD deficiency
  • Participant is pregnant or breast-feeding, or is expecting to conceive during the study.

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
Primaquine PhosphatePrimaquine PhosphateCohort 1 will receive 30 mg of RSPQ (1A) every day for 7 days Cohort 2 will receive 45 mg of RSPQ (1A) every day for 7 days
PlaceboPlaceboCohort 1 will receive placebo (4A) capsules everyday for seven days Cohort 2 will receive placebo (4A) capsules everyday for seven days
RPQ (-) enantiomerRPQCohort 1 will receive 15 mg of RPQ (3A) every day for 7 days Cohort 2 will receive 22.5 mg of RPQ (3A) every day for 7 days
SPQ (+) enantiomerSPQCohort 1 will receive 15 mg of SPQ (2A) every day for 7 days Cohort 2 will receive 22.5 mg of SPQ (2A) every day for 7 days
Primary Outcome Measures
NameTimeMethod
Change in Methemoglobin concentration in blood from baselineDays 0, 3, 5, 7

Change in Methemoglobin concentration in blood from baseline (% hemoglobin)

Secondary Outcome Measures
NameTimeMethod
Change in Hematocrit (%) Compared to baselineDays 0, 3, 5, 7

Change in Hematocrit (%) Compared to baseline

Primaquine Plasma concentration, ng/mLDays 0, 3, 5, 7

Plasma concentrations of parent drug

Change in Hemoglobin (g/dL) Compared to baselineDays 0, 3, 5, 7

Change in Hemoglobin (g/dL) Compared to baseline

Change in AST (U/L) Compared to baselineDays 0, 3, 5, 7

Change in Aspartate aminotransferase (U/L) Compared to baseline; used to monitor liver function

Carboxy- Primaquine Plasma concentration, ng/mLDays 0, 3, 5, 7

Plasma concentrations of carboxy-primaquine metabolite

Primaquine N-carbamoyl-glucuronide Plasma concentration, ng/mLDays 0, 3, 5, 7

Plasma concentrations of Primaquine N-carbamoyl-glucuronide metabolite

Change in Total Bilirubin (mg/dL) Compared to baselineDays 0, 3, 5, 7

Change in Total Bilirubin (mg/dL) Compared to baseline; used to monitor liver function and red cell integrity

Primaquine Orthoquinone Plasma concentration, ng/mLDays 0, 3, 5, 7

Plasma concentrations of Primaquine Orthoquinone metabolite

Change in ALT (U/L) Compared to baselineDays 0, 3, 5, 7

Change in Alanine aminotransferase (U/L) Compared to baseline; used to monitor liver function

Trial Locations

Locations (1)

University of Mississippi

🇺🇸

University, Mississippi, United States

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