Effect of Non-essential Amino Acids on Protein Requirements for Endurance Athletes

Not Applicable

Completed

• Conditions: Increased Metabolic Requirement; Healthy
• Interventions: Dietary Supplement: BCAA-enriched egg protein; Dietary Supplement: large amount of essential amino acids; Dietary Supplement: small amount of essential amino acids; Dietary Supplement: BASE egg protein

• Registration Number: NCT02874638
• Lead Sponsor: University of Toronto

Brief Summary

Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA).

The biological value of protein depends on its amino acid composition. Essential amino acids are believed to have main role on whole body protein metabolism. However, it remains to be determined what role non-essential amino acids (NEAA) may have in regulating protein metabolism and contributing to the increased protein requirements after endurance exercise.

The indicator amino acid oxidation (IAAO) method has clarified the individual amino acid requirement in children, normal healthy adult and clinical populations. However, the IAAO method has never been utilized for assessing the effect of NEAA on protein requirements after endurance exercise.

The objective of the current study is to investigate the importance of NEAA for endurance-trained male subjects.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2016-08-17
• Study First Submitted QC: 2016-08-17
• Study First Posted: 2016-08-22
• Study Start: 2016-09
• Primary Completion: 2017-08
• Study Completion: 2017-12-31
• Status Verified: 2019-03
• Last Update Submitted: 2019-03-28
• Last Update Posted: 2019-04-01

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 7

Inclusion Criteria

• endurance-trained participants who regularly run more than 40 km/week
• Participants who are categorized at least "very good" based on a study by Shvartz & Reibold, in which VO2peak is used as an index. (i.e. the participants whose VO2peak is ≥57 ml/kg/min (18-24 y), ≥54 ml/kg/min (25-29 y), ≥52 ml/kg/min (30-34 y), ≥49 ml/kg/min (35-39 y)
• Ability to complete the 16-km familiarization run in session 2

Exclusion Criteria

• Inability to meet health and physical activity guidelines according to the physical activity readiness questionnaire (PAR-Q+)
• Inability to adhere to any of the protocol guidelines (i.e. alcohol, caffeine consumption)
• Regular tobacco use
• Illicit drug use

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
BCAA-enriched egg protein	BCAA-enriched egg protein	branched-chain amino acid-enriched egg protein
large amount of essential amino acids	large amount of essential amino acids	large amount of essential amino acids made after egg protein, which is equivalent to the amount of amino acids in BCAA
small amount of essential amino acids	small amount of essential amino acids	small amount of essential amino acids made after egg protein, which is equivalent to the amount of essential amino acids in BASE
BASE egg protein	BASE egg protein	0.8 g/kg/d of BASE protein provided as crystalline amino acid made after egg protein.

Primary Outcome Measures

Name	Time	Method
13CO2 (carbon dioxide) excretion rate (μmol/kg/h)	at 8 hours after the end of exercise]	8 hours after exercise followed by ingesting 13C-labelled phenylalanine and one of 5 different amount of amino acids intake, 13CO2 excretion rate is determined by multiplying the enrichment of 13CO2 in breath measured by Mass spectrometry and CO2 production rate measured by metabolic cart.

Secondary Outcome Measures

Name	Time	Method
[13C]phenylalanine oxidation rate (μmol/kg/h)	at 8 hours after the end of exercise	8 hours after exercise followed by ingesting 13C-labelled phenylalanine and one of 5 different amount of amino acids intake, 13CO2 excretion rate is determined dividing 13CO2 excretion rate by urinary enrichment of 13C-phenylalanine in breath measured by Mass spectrometry, and CO2 production rate measured by metabolic cart.