Role of bacteria secreted vesicles in diabetic foot ulcer
Not Applicable
- Conditions
- Health Condition 1: E119- Type 2 diabetes mellitus without complications
- Registration Number
- CTRI/2023/09/057818
- Lead Sponsor
- Science and Engineering research Board (SERB) - Core Research Grant
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- ot Yet Recruiting
- Sex
- Not specified
- Target Recruitment
- 0
Inclusion Criteria
Consenting adult participants that have a confirmed diagnosis of type 2 diabetes based on standard diagnostic criteria such as fasting plasma glucose level (case samples). Healthy consenting adult participants (control samples).
Exclusion Criteria
Participants having an active infection or who are taking immunosuppressive or antimicrobial medication during the time of sample collection or in the week prior to sample collection. Participants who are pregnant or have other conditions such as autoimmune diseases, neutropenia, or cancer will also be excluded to minimize potential confounding factors.
Study & Design
- Study Type
- Observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method Outcome measures for measurement of neutrophil mediated responses to polymicrobial biofilms include neutrophil count, oxidative stress markers (such as measurement of ROS activity of neutrophils), neutrophil function (chemotaxis and phagocytosis assay) and levels of NET generation.Timepoint: Immediately after sample processing
- Secondary Outcome Measures
Name Time Method Outcome measures for RNA sequencing experiments include gene expression analysisTimepoint: Immediately after RNA isolation