Gene Expression Profiles in Chronic Periodontitis

Completed

• Conditions: Chronic Periodontitis
• Interventions: Genetic: Genom-based whole-genome transcriptomic

• Registration Number: NCT02467283
• Lead Sponsor: Kocaeli University

Brief Summary

Current concepts of the causes of periodontitis implicate a bacterial infection as the primary cause of the disease. Several bacterial species residing in a biofilm on tooth surfaces are closely associated with periodontitis. Chronic periodontitis (CP) is initiated and sustained by bacterial plaque, but host defense mechanisms play an integral role in its pathogenesis.

Gene expression profiling is a powerful means of generating comprehensive genome-level data sets on diseases such as cancer, asthma, rheumatoid disorders and periodontitis, and provides significant insight information for these diseases. Gene expression profiling may provide an evidence for involving genes in the pathogenesis of CP and generate further information other than clinical signs and symptoms of CP.

The aim of this study was to identify gene-expression patterns of patients with chronic periodontitis by whole-transcriptome gene-expression analyses.

Detailed Description

Not available

Study Timeline

• Study Start: 2013-02
• Study First Submitted: 2015-06-03
• Study First Submitted QC: 2015-06-05
• Study First Posted: 2015-06-10
• Primary Completion: 2015-08
• Study Completion: 2017-05
• Status Verified: 2018-02
• Last Update Submitted: 2018-02-09
• Last Update Posted: 2018-02-12

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 46

Inclusion Criteria

1. CP patients had at least 20 teeth exhibiting >30% of measured sites with >5mm clinical attachment loss.
2. Had bleeding on probing (BOP) at >50% of the proximal sites.

Exclusion Criteria

1. Had any known systemic diseases that can influence the periodontal status, oral diseases other than chronic periodontitis and ongoing orthodontic therapy.
2. History of hepatitis, HIV infection, or diabetes; requirement for antibiotic prophylaxis, pregnancy or lactation; and long-term use of anti-inflammatory drugs.
3. Should be free of systemic diseases
4. Individuals had to be older than 18 years of age.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Patients with Chronic Periodontitis	Genom-based whole-genome transcriptomic	All subjects were free of systemic diseases and to be included, individuals had to be older than 18 years of age. Chronic periodontitis was diagnosed according to American Academy of Periodontology 1999 Consensus Report.
Control Patients	Genom-based whole-genome transcriptomic	Control subjects had no radiographic evidence of bone loss or any sign of past and present periodontitis. All subjects were free of systemic diseases and to be included, individuals had to be older than 18 years of age.

Primary Outcome Measures

Name	Time	Method
mRNA quantification	2 months

Secondary Outcome Measures

Name	Time	Method
Gen networking map	2 months