Biomarkers of Meats and Potatoes Intake

Not Applicable

Completed

• Conditions: Nutrition Disorders
• Interventions: Other: Meat arm; Other: Starchy foods arm

• Registration Number: NCT04548362
• Lead Sponsor: Professor Lars Ove Dragsted

Brief Summary

This cross-over meal study is a four-way intervention with 12 volunteers randomized at the same time to four meals with fried meats (beef, pork, chicken and amino-acid matched non-meat control) and to four meals with potato products (boiled, chips, fries, and a control with boiled white rice) to search for biomarkers of intake.

Detailed Description

In a two-dimensional, cross-over meal study, 12 healthy volunteers consumed in a randomized sequence four test meals: chicken, pork, beef, and a control made of egg white and pea. At the same time they were independently randomized to four starchy foods to be consumed together with the meats: boiled potatoes, fried potatoes, potato crisps or boiled white rice. Fasting and postprandial urine samples were collected to cover 48 h after each meal and samples were profiled by untargeted LC-ESI-qTOF-MS metabolomics. The profiles following the meal challenges were explored by univariate and multivariate analyses and single and combined markers of intake identified chemically by MS/MS fragmentation experiments and statistically by ROC curves and error rates as well as FDR statistics.

Study Timeline

• Study Start: 2016-02-15
• Primary Completion: 2016-05-30
• Study Completion: 2016-05-30
• Study First Submitted: 2020-09-07
• Study First Submitted QC: 2020-09-11
• Study First Posted: 2020-09-14
• Status Verified: 2021-04
• Last Update Submitted: 2021-04-26
• Last Update Posted: 2021-04-28

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 12

Inclusion Criteria

• healthy

Exclusion Criteria

• non-pregnant, no abuse of alcohol or drugs, no chronic medication except p-pills

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: FACTORIAL

Arm && Interventions

Group	Intervention	Description
Meat meals	Meat arm	This arm contains a 4-way cross-over intervention study with meats
Starchy meals	Starchy foods arm	This arm contains a 4-way cross-over intervention study with

Primary Outcome Measures

Name	Time	Method
Potato intake biomarkers	0-48 hours	To identify intake biomarkers of general potato, fried potato and white rice
meat intake biomarkers	0-48 hours	To identify intake biomarkers of general meat, red meat and poultry

Trial Locations

Locations (1)

Lars Dragsted; 🇩🇰 Frederiksberg, Denmark