Biomarkers of Meats and Potatoes Intake: a Meal Study in Healthy Men and Women - the MEPO Study
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Enrollment
- 12
- Locations
- 1
- Primary Endpoint
- Potato intake biomarkers
Study Overview
Brief Summary
This cross-over meal study is a four-way intervention with 12 volunteers randomized at the same time to four meals with fried meats (beef, pork, chicken and amino-acid matched non-meat control) and to four meals with potato products (boiled, chips, fries, and a control with boiled white rice) to search for biomarkers of intake.
Detailed Description
In a two-dimensional, cross-over meal study, 12 healthy volunteers consumed in a randomized sequence four test meals: chicken, pork, beef, and a control made of egg white and pea. At the same time they were independently randomized to four starchy foods to be consumed together with the meats: boiled potatoes, fried potatoes, potato crisps or boiled white rice. Fasting and postprandial urine samples were collected to cover 48 h after each meal and samples were profiled by untargeted LC-ESI-qTOF-MS metabolomics. The profiles following the meal challenges were explored by univariate and multivariate analyses and single and combined markers of intake identified chemically by MS/MS fragmentation experiments and statistically by ROC curves and error rates as well as FDR statistics.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Factorial
- Primary Purpose
- Basic Science
- Masking
- None
Masking Description
It is not possible to mask foods and for the explorative analysis of results, masking is not required.
Eligibility Criteria
- Ages
- 18 Years to 70 Years (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- Not provided
Exclusion Criteria
- •non-pregnant, no abuse of alcohol or drugs, no chronic medication except p-pills
Arms & Interventions
Meat meals
This arm contains a 4-way cross-over intervention study with meats
Intervention: Meat arm (Other)
Starchy meals
This arm contains a 4-way cross-over intervention study with
Intervention: Starchy foods arm (Other)
Outcomes
Primary Outcomes
Potato intake biomarkers
Time Frame: 0-48 hours
To identify intake biomarkers of general potato, fried potato and white rice
meat intake biomarkers
Time Frame: 0-48 hours
To identify intake biomarkers of general meat, red meat and poultry
Secondary Outcomes
No secondary outcomes reported
Investigators
Professor Lars Ove Dragsted
Professor
University of Copenhagen