Effect of Buccal Fat Pad Derived Mesenchymal Stem Cells Loaded on Allograft and Platelet-rich Plasma in Maxillary Sinus Augmentation
Overview
- Phase
- Phase 1
- Intervention
- Not specified
- Conditions
- Alveolar Bone Loss
- Sponsor
- Shahid Beheshti University of Medical Sciences
- Enrollment
- 20
- Locations
- 1
- Primary Endpoint
- amount of regenerated bone
- Last Updated
- 9 years ago
Overview
Brief Summary
In this study the buccal fat pad derived stem cells (BFPSCs) will be harvested from buccal fat pad tissue of patients receiving maxillary sinus augmentation. In the test group the patients receive a combination of platelet rich fibrin (PRF) and freeze dried bone allograft (FDBA) (SureOss, Hansbiomed, Korea) loaded with BFPSCs. The results will be evaluated by cone beam computed tomography (CBCT)and hematoxylin and eosin staining in 6 months.
Detailed Description
The buccal fat pad derived stem cells (BFPSCs) is harvested from buccal fat pad tissues. The BFPSCs will be loaded on freeze dried bone allograft (FDBA) (SureOss, Hansbiomed, Korea). Furthermore, twenty milliliters of the venous blood is obtained, placed and collected in a sterile tube, and centrifuged (GAC medical) for 14 minutes at 2800 rpm (approximately 400 g). Following centrifugation, three layers are separated in the tube: cellular plasma at the top, platelet rich fibrin (PRF) clot in the middle and red blood cells at the bottom of the tube. The PRF clot is separated by a sterile pincette. The patients in the test group receives BFPSCs loaded on DFDBA with PRF for sinus augmentation and the control group receives combination of PRF and DFDBA (lacking any cells).The results will be evaluated by cone beam computed tomography (CBCT) in 6 months and microscopic evaluation of the biopsies by hematoxylin and eosine staining.
Investigators
Arash Khojasteh
Associate professor
Shahid Beheshti University of Medical Sciences
Eligibility Criteria
Inclusion Criteria
- •patients with posterior maxillary edentulism
- •pneumatized sinus
- •less than 5mm bone height between the alveolar crest and sinus membrane
Exclusion Criteria
- •history of malignancy
- •chemotherapy
- •systemic diseases contradicting dental and surgical treatments
- •conditions or drugs affecting bone remodeling or bone metabolism and connective tissue
- •allergy to collagen
Outcomes
Primary Outcomes
amount of regenerated bone
Time Frame: 6 months
the percent of regenerated bone will be assessed on bone biopsies obtained during implant insertion by H\&E staining by microscope