Comparison of Quantitative Light-Induced Fluorescence (QLF) with traditional methods for assessing the level of microbial infection in root canals of teeth
Not Applicable
- Conditions
- Health Condition 1: K041- Necrosis of pulp
- Registration Number
- CTRI/2024/07/071430
- Lead Sponsor
- Dr Setu
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- ot Yet Recruiting
- Sex
- Not specified
- Target Recruitment
- 0
Inclusion Criteria
Medically healthy patients requiring primary endodontic treatment with diagnosis of pulp necrosis and apical abscess or apical periodontitis
No associated periodontal condition- mobility under physiologic limits, absence of any periodontal pocket
Periapical index score of less than 3
Exclusion Criteria
Previously initiated endodontic treatment
Presence of systemic diseases
Patients who had received NSAIDs or antibiotic treatment within the last three months before
the study.
Teeth with insufficient crown structure preventing rubber dam isolation
Teeth presenting with root resorption (internal/external) or root fracture or perforation.
Periodontally compromised teeth
Study & Design
- Study Type
- Observational
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method 1.Correlation Coefficient between Delta R values of QLF and CFU counts obtained from culture-based analysis in root canal exudates before and after biomechanical preparation in patients diagnosed with pulp necrosis and apical periodontitis/apical abscess. <br/ ><br>2.Correlation coefficient between Delta R values of QLF and microbial counts obtained using real time PCR in root canal exudates before and after biomechanical preparation in patients diagnosed with pulp necrosis and apical periodontitis/apical abscess.Timepoint: After access opening and after biomechanical preparation on the same day
- Secondary Outcome Measures
Name Time Method Quantitative assessment of changes in microbial count in root canal exudate assessed using CFU count and real time PCR before and after biomechanical preparation.Timepoint: After access opening and after biomechanical preparation on the same day