The Effects of Antibiotic Combinations on Stem Cells

Completed

• Conditions: Anti-Bacterial Agents; Stem Cells
• Interventions: Drug: Differentiation Markers

• Registration Number: NCT04713436
• Lead Sponsor: Eskisehir Osmangazi University

Brief Summary

The purpose of this study was to evaluate the indirect effects of antibiotic combinations. SCAP viability, proliferation and apoptosis of the cells were evaluated. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

Detailed Description

The purpose of this study was to evaluate the indirect effects of triple (TAP- ciprofloxacin, metronidazole and minocycline), double (DAP- ciprofloxacin and metronidazole) and modified triple antibiotic pastes (mTAP- ciprofloxacin, metronidazole and cefaclor) on mesenchmal stem cells derived from apical papilla (SCAP). SCAP were isolated from the impacted third molar teeth of the young adults. TAP, DAP and mTAP treatment (14-day medication and 28-day medication) were applied on SCAP and viability, proliferation and apoptosis of the cells were evaluated after four weeks. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

Study Timeline

• Study Start: 2014-01-01
• Primary Completion: 2016-01-01
• Study Completion: 2017-02-01
• Status Verified: 2021-01
• Study First Submitted: 2021-01-11
• Study First Submitted QC: 2021-01-13
• Last Update Submitted: 2021-01-13
• Study First Posted: 2021-01-19
• Last Update Posted: 2021-01-19

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 3

Inclusion Criteria

• Patients with open apex third molar teeth for extraction indication

Exclusion Criteria

• Patients with mature apex third molar teeth for extraction indication

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Triple antibiotic paste(TAP)	Differentiation Markers	ciprofloxacin, metronidazole and minocycline
Modified triple antibiotic paste(mTAP)	Differentiation Markers	ciprofloxacin, metronidazole and cefaclor
Doble antibiotic paste(DAP)	Differentiation Markers	ciprofloxacin and metronidazole

Primary Outcome Measures

Name	Time	Method
Measurment of Cell Viability and Apoptosis	1 year	To evaluate cell viability and apoptosis MuseCell Analyzer Count and Viability kit (Merck Millipore, Taufkirchen, Germany) was used according to the manufacturer's protocol.
Measurment of cell differantiation	1 year	To determine gene expression levels, total RNA of SCAP was extracted using the Ambion PureLink RNA mini kit (Invitrogen, US) and the Roche cell RNA Kit (Penzberg, Germany) according to the manufacturer's instructions.

Trial Locations

Locations (1)

Eskişehir Osmangazi University; 🇹🇷 Eskişehir, Turkey