The Effects of Antibiotic Combinations on Mesenchymal Stem Cells Derived From Apical Papilla
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 3
- 试验地点
- 1
- 主要终点
- Measurment of Cell Viability and Apoptosis
研究概览
简要总结
The purpose of this study was to evaluate the indirect effects of antibiotic combinations. SCAP viability, proliferation and apoptosis of the cells were evaluated. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.
详细描述
The purpose of this study was to evaluate the indirect effects of triple (TAP- ciprofloxacin, metronidazole and minocycline), double (DAP- ciprofloxacin and metronidazole) and modified triple antibiotic pastes (mTAP- ciprofloxacin, metronidazole and cefaclor) on mesenchmal stem cells derived from apical papilla (SCAP). SCAP were isolated from the impacted third molar teeth of the young adults. TAP, DAP and mTAP treatment (14-day medication and 28-day medication) were applied on SCAP and viability, proliferation and apoptosis of the cells were evaluated after four weeks. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 16 Years 至 22 Years（Child, Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Patients with open apex third molar teeth for extraction indication
排除标准
- •Patients with mature apex third molar teeth for extraction indication
研究组 & 干预措施
Triple antibiotic paste(TAP)
ciprofloxacin, metronidazole and minocycline
干预措施: Differentiation Markers (Drug)
Doble antibiotic paste(DAP)
ciprofloxacin and metronidazole
干预措施: Differentiation Markers (Drug)
Modified triple antibiotic paste(mTAP)
ciprofloxacin, metronidazole and cefaclor
干预措施: Differentiation Markers (Drug)
结局指标
主要结局
Measurment of Cell Viability and Apoptosis
时间窗: 1 year
To evaluate cell viability and apoptosis MuseCell Analyzer Count and Viability kit (Merck Millipore, Taufkirchen, Germany) was used according to the manufacturer's protocol.
Measurment of cell differantiation
时间窗: 1 year
To determine gene expression levels, total RNA of SCAP was extracted using the Ambion PureLink RNA mini kit (Invitrogen, US) and the Roche cell RNA Kit (Penzberg, Germany) according to the manufacturer's instructions.
次要结局
未报告次要终点
研究者
Seçil Çalışkan
Assistant Professor
Eskisehir Osmangazi University